This project will combine experiments (Reynolds Lab) and theory (Motter Group) to pilot a study to address three very fundamental questions in cell biology: 1) Does the phenotype of multi-knockout mutants depend on the order of the gene deletions? 2) What defines cell state under a given condition? 3) Can the loss of fitness caused by a gene knockout be systematically recovered by means of additional knockouts? The research will be implemented using E. coli K12 MG1655 as model organism and knockouts on enzyme-coding genes to facilitate modeling. Completion of this research will, in particular, lead to a new paradigm for gene essentiality not captured by current ‘minimal cell’ efforts [Hu16].
|Effective start/end date||8/12/16 → 8/11/18|
- Gordon E. and Betty I. Moore Foundation (#5263.08)