Controlling HIV Latency By Manipulating CycT1 Turnover

Dr. Hultquist will have primary responsibility for oversight of the proteomic and gene editing experiments from conception through execution, analysis, and reporting. He will supervise the work of the Graduate Student, William Cisneros, who will be primarily responsible for conducting the experiments and collecting the data. Dr. Hultquist will supervise and work alongside Mr. Cisneros to optimize the approach, conduct quality controls, and analyze the results. Project work includes preparing reagents, managing samples, conducting all necessary experimental replicates, and validating the required controls. Specific tasks will include: purification and mass spectrometry analysis of CCNT1 under different conditions, CRISPR-Cas9 gene editing in human cell lines and primary cells, and HIV replication experiments.