Our overarching goal is to achieve stable transduction of either HLA-DQB1 or HLA-DRB1 into a multiple knockout CRISPR/Cas9 HLA-class II genome edited cell for the purpose of studying HLA-DQ versus HLA-DR specific immune processes including antigen processing and presentation and T cell activation pathways. To this aim we will: 1. Stably rescue either HLA-DQ or HLA-DR expression in HLA class II knockout cells 2. Demonstrate the ability of the rescued cells to restore T cell allorecognition 3. Demonstrate the ability of the rescued cells to bind either HLA-DQ or HLA-DR specific antibodies The ability to demonstrate stable, genome edited, 3D appropriate conformation expression of isolated HLA-DQ or HLA-DR molecules, with physiologic characteristics, will provide the required infrastructure to proceed with functional studies interrogating pathways activated by either of these molecules as they participate in different immune processes
|Effective start/end date||1/1/21 → 12/31/24|
- Vanguard Charitable Endowment Program (Letter 12/2/2020)
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