Development of Novel Anti-Biofilm Compounds for Treating Chronic Wounds

Full thickness dermal wounds will be created on the ventral ear down to the perichondrium of New Zealand White rabbits. Wounds will be infected S. aureus UAMS-1, P. aeruginosa PAO1, or a mixture of the two bacteria three days postoperative. Bacteria will be allowed to proliferate under Tegaderm dressing and then treated on postoperative day 4 with topical antimicrobials (2% mupirocin for S. aureus and 0.3% ciprofloxacin for P. aeruginosa) to eliminate planktonic bacteria. Treatments of H10, antimicrobial, or the combination will be applied on days 5, 6, 8, and 10. On day postoperative day 12, the experiment will be terminated and wounds will be harvested for various analyses.