Our proposal seeks funding for the acquisition of an Electrical/Asymmetric Flow Field-Flow Fractionation (E/AF4) system with static and dynamic light scattering detection. This equipment provides state-of-the-art capabilities for integrated separation and characterization of broad range of materials used in modern biomedical research including proteins and multi-protein complexes, extracellular vesicles, viruses and vaccines, synthetic nanoparticles and polymeric nanocarriers. The system has analytical and preparative capabilities for high-resolution separation of particles 1nm-1m in size and provides precise measurements of particle size, mass, and zeta potential; shape and polydispersity indices can also be calculated from these measurements. These capabilities are required by many research projects currently underway at Northwestern University (including, but not limited to the 14 NIH-funded projects described in detail in this application). These are projects seeking to understand various aspects of genomic organization and function, structure-activity studies aiming to identify and characterize new molecular targets for anti-cancer drugs as well as nanotechnology research projects pursuing innovative approaches for targeted therapies. A total of 10 prospective major users (9 with active NIH funding) and 5 minor users (3 with active NIH funding) are requesting these capabilities. This type of equipment does not exist at Northwestern University or in any core facility in the Chicago area. The instrument will be integrated in the Keck Biophysics Facility a well-known resource of shared equipment and technical expertise at Northwestern. The facility has a 24-year track record of keeping a diverse collection of high-end research instruments operational and accessible and facilitating the production of large amounts of first-rate science
|Effective start/end date||8/1/22 → 7/31/23|
- National Institutes of Health (NOT SPECIFIED)
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