Genetics and quantum chemistry as tools for unknown metabolite identification

Description of work to be performed at Northwestern University (Andersen lab): We will contribute to Specific Aim 1. To complete Aim 1, we will preform the following steps: • Aid in the design and implementation of sample preparation for wild isolates and deletion strains • Create a single large batch of E. coli food to use for all metabolite studies • Aid in the design and implementation of C. elegans sorting experiments to get pure samples for metabolite extractions • Perform genome-wide association studies for metabolites across a large collection of wild C. elegans strains • Relate these GWA mapping results to deletion studies performed by other members of the consortium