Impact of Antibody Effector Function Diversity on Antiviral Activity In Situ

The Hope laboratory will complete the detailed analysis of the distribution and localization of five groups of mixtures of three differentially labelled fluorescent antibodies. These combinations will be evaluated in groups of three animals. The Hope laboratory will be responsible for the fluorescent labeling and characterization of these antibodies. The Hope laboratory will also be responsible for the characterization of antibody distribution utilizing different imaging modalities. We will also do the quantification and analysis of the distribution of the different antibodies in vivo. Additionally, we will lead studies of the interaction of antibodies, host effector cells, and SHIV infected cells guided to sites of viral replication utilizing correlative PET/CT as part of Specific Aim 3. We will work with PPG team members to correlate the outcome of these studies with the protective functions of the monoclonal antibodies to be evaluated.