Investigation of ALS caused by mutant CHCHD10

Project: Research project

Description

Some of the most robust advances in the understanding of amyotrophic lateral sclerosis (ALS), a multi-etiologic and fatal disorder of the nervous system, have come from the identification of the genetic basis of a subgroup of ALS cases. In this subgroup of ALS (familial ALS or FALS), the disease is inherited as a familial trait. Identification of a single mechanism of disease across all of ALS has been elusive, but notable amongst proposed common mechanisms is mitochondrial dysfunction. However, till recently, no direct robust etiological link between mitochondrial genes, nuclear or mitochondrial, had been found. Recently, others and we found that mutations in a gene called CHCHD10 causes ALS and additional phenotypes. Some other ALS genes, including VCP, HNRNPA1, SQSTM1 and OPTN are also associated with other phenotypes besides ALS. CHCHD10 and its protein product are not well studied. To investigate how a mutation in CHCHD10 may cause ALS, we have collected or developed promising reagents and assembled collaborators and laboratory personnel with specialized skills in genetic engineering, rodent models, cell biology, CRISPR/Cas9 gene editing, mitochondrial function, redox response, induced pluripotent stem cell (iPSC) derived motor neurons and primary motor neurons to carry out the proposed experiments in my laboratory and the laboratories of collaborators.
We will investigate the pathology and behavior of a new transgenic mouse model we have engineered to overexpress the mutant (R15L) human CHCHD10 gene. Preliminary study shows axonal pathology in the spinal cord and brain of this mouse with periodic beaded axonal swellings harboring mitochondria. Additional more precise models will also be developed. To determine what aspect of mitochondrial function or morphology is affected by mutant CHCHD10 we will screen mitochondrial function of energetics, apoptosis, redox studies, calcium signaling and electron microscopy analysis. We will identify the binding protein partner(s) of CHCHD10 protein to understand the role of CHCHD10 in the mitochondrial biology. On completion, our study would provide a clearer understanding of the central defect(s) in this form of ALS and potentially a more granular understanding of mitochondrial defect generalizable across ALS. The study will also allow the identification of potential molecular targets for rational therapy and/or prevention of ALS.
StatusActive
Effective start/end date9/1/168/31/21

Funding

  • National Institute of Neurological Disorders and Stroke (5R01NS099638-04)

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Amyotrophic Lateral Sclerosis
Motor Neurons
Oxidation-Reduction
Clustered Regularly Interspaced Short Palindromic Repeats
Pathology
Genes
Phenotype
Laboratory Personnel
Induced Pluripotent Stem Cells
Mutation
Genetic Engineering
Calcium Signaling
Mitochondrial Genes
Nervous System Diseases
Transgenic Mice
Cell Biology
Rodentia
Spinal Cord
Electron Microscopy
Carrier Proteins