Mapping the 3D architecture of native human replisomes

  • Yang, Hongbo (PD/PI)

Project: Research project

Project Details

Description

Dr. Yang will collaborate with Dr. Gilbert (the PI) at the Florida State University as a Co- Investigator for the proposed project. Dr. Yang’s group will collect all of the Bionano Saphyr data proposed in the grant application. We estimate that this will entail approximately 1000-fold coverage of the human genome each year. For this, we will receive frozen cell pellets, prepare samples and run approximately 12 Saphyr flow cells per year. We will collect the data and run the Bionano data analysis pipeline to localize fluorescent incorporation signals on mapped DNA molecules. We will send this data to your lab for identification of replication tracks and analysis of origin and fork locations.
StatusFinished
Effective start/end date8/2/195/31/21

Funding

  • Florida State University (R02196//5R01HG010658-02)
  • National Human Genome Research Institute (R02196//5R01HG010658-02)

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.