Mapping the 3D architecture of native human replisomes

Project: Research project

Project Details


Dr. Yang will collaborate with Dr. Gilbert (the PI) at the Florida State University as a Co-
Investigator for the proposed project. Dr. Yang’s group will collect all of the Bionano
Saphyr data proposed in the grant application. We estimate that this will entail
approximately 1000-fold coverage of the human genome each year. For this, we will
receive frozen cell pellets, prepare samples and run approximately 12 Saphyr flow cells
per year. We will collect the data and run the Bionano data analysis pipeline to localize
fluorescent incorporation signals on mapped DNA molecules. We will send this data to
your lab for identification of replication tracks and analysis of origin and fork locations.
Effective start/end date8/2/195/31/21


  • Florida State University (R02196//5R01HG010658-02)
  • National Human Genome Research Institute (R02196//5R01HG010658-02)


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