Maximizing Quantitative Structural Information from High-Throughput RNA Structure Probing

Our long-term goal is to address these existing limitations. Here we propose to establish the feasibility of methods to more accurately model RNA structure by extracting quantitative structural information from chemical probing experiments through (1) creating an external RNA standard to normalize data between experiments and across labs and (2) designing and constructing a panel of benchmark RNAs to begin to quantitatively understand the structural basis of experimental reactivity.