Molecular decisions on the move: Making movies of RNA switches using big data

Project: Research project

Project Details


RNAs fold cotranscriptionally as they are synthesized, however technical barriers have limited our understanding of how RNA folding directs RNA function. We previously developed a method for the high-resolution measurement of RNA structure during synthesis. In this proposal, we seek to (1) simplify the cotranscriptional SHAPE-Seq protocol, (2) expand the capabilities of cotranscriptional SHAPE-Seq to allow the detection of RNA structure subpopulations, and (3) use cotranscriptional SHAPE-Seq to uncover the role of cotranscriptional RNA folding in controlling gene regulation using the ZTP riboswitch as a model system.
Effective start/end date9/1/178/31/20


  • Arnold and Mabel Beckman Foundation (Letter 2/17/2017)


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