Monocyte-derived alveolar macrophages drive inflammatory response to lung ozone exposure

Dr. Misharin will direct sample processing, sequencing, analysis, data sharing, including deposition into controlled and public repositories as wells fiscal and scientific oversight. Dr. Misharin’s team will receive human and mouse samples from Dr. Tighe and perform processing according to established protocol to perform single cell transcriptomic profiling, including library preparation, sequencing, data analysis and sharing. Dr. Misharin with work with Dr. Tighe on data analysis and publication. According to the current plan Dr. Tighe will recruit 5 subjects in year 1, and 10 subjects in years 2-5 of the proposal, collecting paired samples before and after ozone exposure. These samples will be shipped to Northwestern and processed by Dr. Misharin’s team. Additionally, ~20 mouse samples will be shipped to Northwestern team using the same protocols over the course of year 1-3.