Mucosal predictors of HIV acquisition

Project: Research project

Project Details

Description

The Hope lab has extensive experience in microscopic
imaging technology to identify the first SIV target cells in the FGT, as well as to identify the earliest
sites (foci) and cells infected after vaginal inoculation. Antibodies will be used to stain for in vivo
HIV target cells as described, including CD4+ T cells (CD3+CD4+), macrophages (CD14+CD4+),
co-receptors (CCR5,CXCR4,CCR6), integrins (47), and activation status (HLA-DR, CD38,
Ki67). CAPRISA biomaker proteins will be evaluated at these sites by immunohistochemistry and
immunofluorescense at foci of infection, and immediately adjacent sections where there is an
absence of infected foci. Differences in protein expression will be performed by standard t-test of
flurorescent density as measured by microscopy. We will assess whether CAPRISA HIV risk
biomarkers co-localize in the FGT in vivo with increased target cell density or frequency, and
underlies HIV susceptibility.
StatusFinished
Effective start/end date11/1/1710/31/18

Funding

  • University of Manitoba (AGREEMENT 06/19/18)
  • Canadian Institutes of Health Research (AGREEMENT 06/19/18)

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