Multi-Tissue Craniofacial Engineering using 3D-BMP9-Notch-Synergized Graphene Citrate Composite Scaffolds

Northwestern University will perform the biomaterial- and scaffold-related research activities associated with Specific Aim 2 of the proposal: “Develop structural composite scaffolds that can be customized to fit and regenerate a critical-sized skin and bone calvarial defect in a rodent model.” These experiments will include the synthesis and fabrication of hydrogel and 3D-printed scaffolds for bone and skin regeneration. In vivo biocompatibility and bone tissue formation will also be performed in rodent models. Tissue samples will be processed for histology and histomorphometry to assess inflammation, foreign body response, new tissue formation, and other markers associated with the safety of the scaffolds.