Multifunctional Printed Scaffolds for Enhancing Hepatocyte Viability and Function

  • Shah, Ramille Nirav (PD/PI)

Project: Research project

Project Details

Description

The proposed research aims to use multifunctional 3D bioprinted scaffolds incorporating bioactive agents
such peptide nanostructures and support cells to enhance the viability and function of hepatocytes for liver
tissue engineering. The lack of liver donors for patients with end stage liver disease (ESLD) is a major
healthcare obstacle. Developing organ replacements or functional liver units using tissue engineering
strategies as an alternative treatment is a promising possibility to alleviate this significant need. The interplay
between microenvironmental cues and cell behavior in liver tissue engineering, however, is still not well
understood. This research hopes to establish and understand trends in 3D scaffold design and bioactive agent
delivery to start to identify key components in the microenvironment that can enhance liver cell function and
normal liver tissue formation both in vitro and in vivo. The hypothesis is that the viability and function of
hepatocytes can be significantly enhanced by: 1) optimizing scaffold architecture, which can alter hepatocyte
aggregation and cellcell
contact; 2) including functional moieties for growth factor delivery via selfassembling
peptide amphiphile (PA) nanofibers; and 3) coculturing
stromal cells and hepatocytes with 3D spatial control.
Small “liver units” will be created using 3D bioplotted scaffolds of varying pore size and geometry, peptidebased
nanostructures and growth factors for bioactive signaling, and liver cells (primary hepatocytes, induced
pluripotent stem cellderived
hepatocytes, and stromal cells) that are spatially patterned in 3D to change
microenvironmental cues and determine what conditions promote optimal hepatocyte viability and
function. Materials characterization, in vitro evaluation of viability, proliferation, and function, as well as in vivo
assessment of angiogenesis, scaffold degradation, and tissue synthesis will be performed to evaluate the
potential of these multifunctional scaffolds for liver tissue engineering. The results from this research will
provide the necessary preliminary data for securing R01 funding that will help establish Dr. Shah as an
independent investigator in the liver tissue engineering field.
StatusFinished
Effective start/end date8/1/167/31/18

Funding

  • National Institute of Diabetes and Digestive and Kidney Diseases (4K01DK099454-04)

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