Novel tools for in vitro electrophysiology and neurotrauma modeling

The Rogers group will design, fabricate and test various novel two and three dimensional sensor arrays for interfaces to cells and organoids, in close collaboration with the broader team. The Huang group will lead the design, optimization and analysis part of the proposed research project on in vitro neurotrauma study. We will develop mechanics design principles for the high performance flexible multielectrode arrays (MEAs) for electrical sensing and actuation as well as the mechanical system to apply biofidelic trauma to brain organoids in adherent culture conditions. Based on our extensive expertise in the mechanics of stretchable and flexible electronics, and the deterministic three-dimensional assembly, we will continue (1) to optimize the two-dimensional, flexible MEAs to withstand large-displacement indentation due to biofidelic trauma, (2) to study the impact of Matrigel layer to the flexible MEAs, (3) to design and optimize three-dimensional MEAs with more complex geometries, more sensing electrodes and better arranged strain gages for enhanced capabilities to contain and monitor spheroid brain organoids, (4) to refine our finite-element models to capture characteristic behaviors of the three-dimensional MEA and the confined, adherent brain organoids during high-speed impact loading, (5) to help optimize the design and layout of the proposed spheroid injury apparatus, and other necessary aspects in order to successfully carry out the proposed research work.