The goal of this research project is the development of synthetic probes with photoactivatable fluorescence to image multiple intracellular components in live cells with spatial resolution at the nanometer level. This challenging objective demands the identification of viable structural designs to enable fluorescence photoactivation with illumination conditions that avoid autofluorescence from and photodamage to live specimens as well as strategies to connect photoactivatable probes to intracellular targets in live cells selectively with labeling conditions that ensure negligible perturbations of cell functions and structures. In particular, the specific aims of this proposal are focused on the development of a mechanism to photoactivate bright far-red fluorescence with infinite contrast under green illumination and, eventually, of an entire family of switchable synthetic probes with partially-resolved emissions based on this photoactivation strategy. These research activities demand a significant amount of chemical synthesis to modify the structures of photoswitchable BODIPY–oxazine dyads, developed in the laboratories of the principal investigator over the course of the previous funding cycles, detailed spectroscopic analyses to characterize the photochemical and photophysical properties of the modified compounds, the labeling of model cells lines with these molecular probes and systematic imaging experiments of the resulting preparations.
|Effective start/end date||8/15/20 → 7/31/23|
- University of Miami (OS00000274//1954430)
- National Science Foundation (OS00000274//1954430)