Research for this study performed at Northwestern University will be under the direction of Dr. Alan Hauser, who will serve as co-investigator of the study. Northwestern University will provide all work related to sequencing the genomes of the Pseudomonas aeruginosa isolates described in this proposal and will analyze these sequences to extract accessory genomic elements. In addition, the bioinformatics analysis to link specific accessory genomic elements to phenotypes of sustained eradication vs. lack of sustained eradication and to phenotypes of slow vs. rapid decline in FEV1 will also be performed at Northwestern University. Finally, some accessory genomic element deletion mutants may be generated at Northwestern. Retrieval of the relevant P. aeruginosa isolates, mouse experiments, generation of other accessory genomic element deletion mutants, and analysis of results will be performed at the University of Washington. The goal of this proposal is to characterize these Pseudomonas aeruginosa isolates to link specific accessory genomic elements to features of progressive pulmonary disease in cystic fibrosis.
|Effective start/end date||1/1/16 → 12/31/19|
- University of Washington (UWSC8969//SINGH15U0)
- Cystic Fibrosis Foundation (UWSC8969//SINGH15U0)