This proposal uses a toolbox of viral techniques to trace and functionally characterize the amacrine and bipolar cells that provide input to specific RGCs in both the rod dominant mouse and cone dominant ground squirrel retinas. In three specific aims, our goals are to: 1) use a trans-synaptic rabies virus that expresses GFP to map the direct bipolar and amacrine cell inputs to genetically targeted RGCs in the mouse retina; 2) use a trans-synaptic rabies virus that expresses the Ca2+ indicator protein GCaMP6 to study the functional connections between RGCs and their direct inputs from bipolar and amacrine cells in the mouse retina; and, 3) identify and functionally characterize the inner retinal circuits responsible for blue/green color opponent vision in the ground squirrel. Our work will define the wiring and functions of specific inner retinal circuits in health and provide the background for understanding circuit changes that are known to occur following photoreceptor degeneration, whether from genetic or age-onset disease.
|Effective start/end date||9/1/21 → 6/30/25|
- National Eye Institute (5R01EY018204-12)
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