NU will be responsible for development of PWS instrumentation for the analysis of cellular nanoarchitecture. NU will also be responsible for the development of standard operating procedures for PWS analysis including 1) identification of the cellular location of the most pronounced increase in the disorder strength (cell nucleus versus cytoplasm), 2) optimization of cell processing including fixation and staining, 3) development of the optimal statistics characterizing increase in the disorder strength. These studies will be performed in vitro on cell lines (HT29 and their genetically modified variants). The PWS instrumentation will be made available to investigators at NUH who will use it for analysis of cells brushed from human subjects. NU will also be responsible for the statistical analysis of correlations among nanoarchitectural and immunohistochemical markers.
|Effective start/end date||7/1/12 → 1/31/13|
- NorthShore University HealthSystem Research Institute (EH13-052-S1//6U01CA111257-08)
- National Cancer Institute (EH13-052-S1//6U01CA111257-08)