Student Award in support of Kelly Wun, for "Modulation of Neointimal Hyperplasia after Arterial Injury by Microbe-Derived Propionate"

Project: Research project

Project Details

Description

Annually, 3 million Americans undergo balloon angioplasty/stenting or arterial bypass for severe atherosclerosis, but rate of treatment failure by restenosis is as high as 70% at 2 years. Restenosis is caused by neointimal hyperplasia, a cellular proliferative process initiated and potentiated by inflammation.
Short chain fatty acids (SCFA), products of bacterial fermentation of dietary fiber, have known anti-inflammatory and anti-proliferative effects. Butyrate, propionate, and acetate comprise over 80% of gut-derived SCFA. My project will investigate the effect of propionate on neointimal hyperplasia development. First, I will treat wild-type mice in four different ways prior to performing femoral artery wire injury: control drinking water, vancomycin-treated water, propionate-treated water, and vancomycin+propionate-treated water. I will examine neointimal hyperplasia development, perform serum analysis, and microbiome analysis of stool pellets in each group. I will analyze gene and protein expression of vascular smooth muscle cells treated with and without propionate to better understand propionate’s mechanistic effect on these cells.
Specific methods will be:
1. Mouse femoral artery wire injury model of neointimal hyperplasia
2. Genomic DNA purification of fecal samples
3. 16S sequencing and analysis of fecal microbial taxonomy and diversity
4. GC-MS of serum and stool for SCFA
5. Histological analysis of mouse femoral arteries
6. Protein and RNA isolation from mouse aortic vascular smooth muscle cells
7. qRT-PCR
8. Western blotting and immunoprecipitation
StatusFinished
Effective start/end date5/1/174/30/18

Funding

  • Society for Vascular Surgery Foundation (Agreement 5/10/17)

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