T Cell Receptor Repertoire Analysis as a Biomarker for Predicting Graft Rejection in Transplant Recipients

Project: Research project

Description

The objective of the current proposal is to develop a new sensitive technology using the ImmunoSEQ assay platform to prospectively assess the donor-reactive T cell repertoire in non-invasive blood (and/or urine in kidney transplant) samples. It is our hypothesis that comprehensive profiling of donor-reactive T-cell repertoire can be made by using recipient pre-transplant mixed lymphocyte reaction (MLR) versus the donor and the immunoSEQ platform, and that detection of the same donor-reactive clones in post-transplant blood (urine pellets), and/or biopsies is diagnostic of acute rejection. We further posit that longitudinal tracking of pre-identified donor-reactive T cell clones is predictive of post-transplant rejection, or predict progression from subclinical to acute rejection, and that this can be monitored non-invasively in the post-transplant blood (and/or urine pellets) rather than in biopsies.
StatusActive
Effective start/end date9/30/179/29/20

Funding

  • U.S. Army Medical Research and Materiel Command (W81XWH-17-1-0679)

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Graft Rejection
T-Cell Antigen Receptor
Biomarkers
Tissue Donors
Urine
T-Lymphocytes
Transplants
Clone Cells
Biopsy
Mixed Lymphocyte Culture Test
Transplant Recipients
Technology
Kidney