The Neuroproteomics and Neurometabolomics Center on Cell-Cell Signaling

The role of the NU team in the Bioinformatics and Systems Biology Core component of the Neuroproteomics and Neurometabolomics Center is to analyze high resolution LC-MS datasets obtained by the Center for users. The work will use data processing resources we have previously published to identify, characterize and quantify unique proteins within the samples. Our resources include a full suite of bioinformatics software tools which can applied to analyze “Bottom-Up” and “Middle-Down” shotgun datasets where proteins are digested to various extents either in vivo or in vitro prior to MS analysis. Our tools also support analysis of “Top-Down” data where intact proteins introduced into the mass spectrometer are subjected to mass analysis and structural characterization by tandem MS (MS/MS). Our work is in close alignment with the Sampling and Separation Core and the Molecular Profiling and Characterization Core within the Center and we will collectively design experiments that apply methods most appropriate for each sample after consultation with each user.