The Role of Placental Maternal Vascular Underperfusion in Neonatal Pulmonary Hypertension

Project: Research project

Project Details

Description

Dr. Misharin will provide his oversight and expertise in the humanized mouse model experiments, including engraftment of human monocytes into newborn mice and interpretation of the lung findings. He will also provide his expertise in conducting in vitro experiments and gene expression studies of monocyte subsets. He will help design and conduct specific workflows, interpretation of the data that pertain to monocyte/macrophage functions in lung injury and repair. Dr. Misharin will also serve as the Northwestern site PI to address any administrative issues related to the grant after transfer.He will meet regularly with Dr. Mestan and her team via remote meetings, and assist with preparation of results for presentation and publication. Dr. Swaminathan, Director of the Lurie Cancer Center Flow Cytometry Core at NU, will be responsible for adjusting the workflows and methods pertaining to flow cytometry analysis, fluorescence activated cell sorting (FACS), isolation and archive of cord blood monocytes upon transfer of the protocols to UCSD. She will continue to be involved in the training of new research personnel and oversee validation of new workflows as they are transferred to UCSD. She will continue to review flow cytometry outputs, and assist the UCSD investigators in validating the flow cytometry, gating methods and FACS procedures for human and mouse samples. She will meet regularly with the PI and her team via remote meetings, and assist with preparation of results for presentation and publication.
StatusFinished
Effective start/end date3/8/221/31/24

Funding

  • University of California, San Diego (705214 // 7R01HL139798-06)
  • National Heart, Lung, and Blood Institute (705214 // 7R01HL139798-06)

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