Tissue eosinophil subtypes in homeostasis and inflammation

Project: Research project

Project Details

Description

There are potentially multiple eosinophil subsets with different roles in health in disease. We have a long standing interest in the biology of tissue resident eosinophils. Our research group was the first to publish that there are resident eosinophils in murine lung tissue and that eosinophils in mouse models of asthma exhibit plastic phenotypes (Abdala-Valencia et al. 2016). Reports immediately followed that confirmed our observations and showed that lung resident Siglec-F(+)CD11c(-) cells represent a distinct population of eosinophils (Mesnil et al. 2016). We since realized that, even in homeostasis, there is remarkable heterogeneity of tissue eosinophils in different organs and tissue states. Among these, we identified homeostatic eosinophil subsets in normal murine lungs during postnatal development. We summarized the current knowledge of tissue eosinophil heterogeneity, emerging markers, and nomenclature in our recent review (Abdala-Valencia et al. 2018). However, our understanding of eosinophil heterogeneity is far from complete. Tissue characterization of eosinophil markers is currently lacking, especially in biologically relevant contexts. Our group is pioneering the use of single cell RNA-seq and tissue-driven functional assays to study eosinophil subtypes and, therefore, directly tackle this problem. We have already made a significant advancement in the ability to detect eosinophils by single cell sequencing, as well as gained insights into their biology and tissue-relevant markers. Further and complete resolving of tissue eosinophil subtypes, as well as the identification of tissue markers, will allow us to elucidate the beneficial vs. pathogenic functional phenotypes of these cells in varying tissue and disease contexts.
StatusActive
Effective start/end date5/20/215/31/24

Funding

  • GlaxoSmithKline LLC (Agmt 3000037272)

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