α-linolenic acid inhibits human renal cell carcinoma cell proliferation through PPAR-γ activation and COX-2 inhibition

Lijun Yang, Jianlin Yuan, Liwen Liu, Changhong Shi, Longxin Wang, Feng Tian, Fei Liu, He Wang, Chen Shao, Qiang Zhang, Zhinan Chen, Weijun Qin*, Weihong Wen

*Corresponding author for this work

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

ω-3 fatty acids have potential anticancer effects, and consuming food rich in ω-3 fatty acids reduces the human renal cell carcinoma (RCC) risk. However, the direct effect of ω-3 fatty acids on RCC in vitro is unknown. In the present study, the effects of α-linolenic acid (ALA), an ω-3 fatty acid, were observed on cell proliferation in the RCC cell line OS-RC-2. The activity and gene expression levels of peroxisome proliferator-activated receptor-γ (PPAR-γ) and cyclooxygenase-2 (COX-2) in the OS-RC-2 cells were measured by ELISA and real-time RT-PCR, respectively, following ALA treatment. ALA (20-80 μM) dose-dependently suppressed the proliferation of the OS-RC-2 cells. PPAR-γ activity and gene expression were significantly increased by ALA at 20 and 40 μM. COX-2 activity and gene expression levels were significantly decreased by ALA from 20 μM. Use of purely the PPAR-γ agonist, rosiglitazone, decreased the proliferation of the OS-RC-2 cells, while ALA induced further suppression of cell proliferation in the presence of rosiglitazone. The COX-2 inhibitor N-(3-Pyridyl)indomethacinamide induced further suppression of cell proliferation in the presence of rosiglitazone. N-(3-Pyridyl)indomethacinamide also suppressed the proliferation of the OS-RC-2 cells. In the presence of N-(3-Pyridyl)indomethacinamide, ALA and rosiglitazone further inhibited OS-RC-2 cell proliferation. In conclusion, ALA inhibits the cell proliferation of the OS-RC-2 human RCC cell line. PPAR-γ activation and COX-2 inhibition serve as two signaling pathways for the inhibitory effects of ALA on RCC cell proliferation.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalOncology Letters
Volume6
Issue number1
DOIs
StatePublished - Jul 2013

Keywords

  • Cell proliferation
  • Cyclooxygenase-2
  • Peroxisome proliferator-activated receptor-γ
  • Renal cell carcinoma
  • α-linolenic acid

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Yang, L., Yuan, J., Liu, L., Shi, C., Wang, L., Tian, F., Liu, F., Wang, H., Shao, C., Zhang, Q., Chen, Z., Qin, W., & Wen, W. (2013). α-linolenic acid inhibits human renal cell carcinoma cell proliferation through PPAR-γ activation and COX-2 inhibition. Oncology Letters, 6(1), 197-202. https://doi.org/10.3892/ol.2013.1336