αE-catenin regulates actin dynamics independently of cadherin-mediated cell-cell adhesion

Jacqueline M. Benjamin, Adam V. Kwiatkowski, Changsong Yang, Farida Korobova, Sabine Pokutta, Tatyana Svitkina, William I. Weis, W. James Nelson

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

αE-catenin binds the cell-cell adhesion complex of E-cadherin and β-catenin (β-cat) and regulates filamentous actin (F-actin) dynamics. In vitro, binding of αE-catenin to the E-cadherin-β-cat complex lowers αE-catenin affinity for F-actin, and αE-catenin alone can bind F-actin and inhibit Arp2/3 complex-mediated actin polymerization. In cells, to test whether αE-catenin regulates actin dynamics independently of the cadherin complex, the cytosolic αE-catenin pool was sequestered to mitochondria without affecting overall levels of αE-catenin or the cadherin-catenin complex. Sequestering cytosolic αE-catenin to mitochondria alters lamellipodia architecture and increases membrane dynamics and cell migration without affecting cell-cell adhesion. In contrast, sequestration of cytosolic αE-catenin to the plasma membrane reduces membrane dynamics. These results demonstrate that the cytosolic pool of αE-catenin regulates actin dynamics independently of cell-cell adhesion.

Original languageEnglish (US)
Pages (from-to)339-352
Number of pages14
JournalJournal of Cell Biology
Volume189
Issue number2
DOIs
StatePublished - Apr 19 2010

ASJC Scopus subject areas

  • Cell Biology

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