β-adrenergic agonists regulate Na-K-ATPase via p70S6k

Liuska Pesce, Alejandro Comellas, Jacob I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We have recently reported that the β-adrenergic agonist isoproterenol regulates the alveolar epithelial cell Na-K-ATPase via MAPK/extracellular signal-regulated kinase and rapamycin-sensitive pathways. Here we report that isoproterenol phosphorylated the protein S6 kinase (p70S6k) in alveolar epithelial cells, which was inhibited by both rapamycin and the MEK1/2 inhibitor U-0126. In alveolar epithelial cells transfected with a p70 S6k dominant negative construct, isoproterenol did not increase Na-K-ATPase total protein expression, whereas in cells transfected with a rapamycin-resistant mutant, the isoproterenol-mediated increase in Na-K-ATPase was not prevented by rapamycin. Accordingly, we provide here first evidence that isoproterenol regulates Na-K-ATPase via p70S6k in alveolar epithelial cells.

Original languageEnglish (US)
Pages (from-to)L802-L807
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume285
Issue number4 29-4
DOIs
StatePublished - Oct 1 2003

Keywords

  • Isoproterenol
  • Lung
  • Mitogen-activated protein kinase
  • Phosphatidylinositol 3-kinase
  • Rapamycin

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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