Abstract
We have recently reported that the β-adrenergic agonist isoproterenol regulates the alveolar epithelial cell Na-K-ATPase via MAPK/extracellular signal-regulated kinase and rapamycin-sensitive pathways. Here we report that isoproterenol phosphorylated the protein S6 kinase (p70S6k) in alveolar epithelial cells, which was inhibited by both rapamycin and the MEK1/2 inhibitor U-0126. In alveolar epithelial cells transfected with a p70 S6k dominant negative construct, isoproterenol did not increase Na-K-ATPase total protein expression, whereas in cells transfected with a rapamycin-resistant mutant, the isoproterenol-mediated increase in Na-K-ATPase was not prevented by rapamycin. Accordingly, we provide here first evidence that isoproterenol regulates Na-K-ATPase via p70S6k in alveolar epithelial cells.
Original language | English (US) |
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Pages (from-to) | L802-L807 |
Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
Volume | 285 |
Issue number | 4 29-4 |
DOIs | |
State | Published - Oct 1 2003 |
Keywords
- Isoproterenol
- Lung
- Mitogen-activated protein kinase
- Phosphatidylinositol 3-kinase
- Rapamycin
ASJC Scopus subject areas
- Physiology
- Pulmonary and Respiratory Medicine
- Physiology (medical)
- Cell Biology