γδ T cells support gut Ag-reactive colitogenic effector T-cell generation by enhancing Ag presentation by CD11b+ DCs in the mesenteric LN

Jeongsu Do, Anabelle Visperas, Michael L. Freeman, Eunjung Jang, Sohee Kim, Bernard Malissen, Booki Min*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

T cells expressing the γδ TCR are dominant T-cell subsets in the intestinal immune system. We previously demonstrated that γδ T cells play important roles in augmenting Th17-type colitogenic immune responses in a T-cell-induced colitic inflammation model. However, its underlying mechanism remains poorly understood. In this study, an in vitro coculture system using effector T cells enriched in gut Ag-reactive cells was employed as a readout tool to search for gut Ag presenting APCs. We found that the presence of γδ T cells dramatically enhances gut Ag presentation within the mLN in mice. Gut Ag presentation by CD11b+ DC subsets was particularly controlled by γδ T cells. Interestingly, γδ T-cell entry to the lymph nodes was essential to improve the Ag presentation. Therefore, our results highlight that γδ T cells play a previously unrecognized role to support colitogenic immunity by regulating gut Ag presentation in the draining LN.

Original languageEnglish (US)
Pages (from-to)340-346
Number of pages7
JournalEuropean Journal of Immunology
Volume46
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Keywords

  • Colitis
  • DCs
  • Gut Ag
  • Presentation
  • γδ T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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