TY - JOUR
T1 - 1α,25-dihydroxyvitamin D3 nuclear receptors in the pituitary
AU - Gelbard, Harris A.
AU - Stern, Paula H.
AU - U'Prichard, David C.
PY - 1980
Y1 - 1980
N2 - Specific binding of 1α,25-dihydroxyvitamin D3 was found in nuclear and cytosol fractions of the bovine pituitary. For nuclear binding, the dissociation constant was 0.1 nanomole per liter, and maximum binding was 104 femtomoles per milligram of protein. In competition studies, 25-hydroxyvitamin D3 was 300 times weaker than 1α,25-dihydroxyvitamin D3. The existence of high-affinity sites supports a physiologic role for 1α,25-dihydroxyvitamin D3 in the pituitary.
AB - Specific binding of 1α,25-dihydroxyvitamin D3 was found in nuclear and cytosol fractions of the bovine pituitary. For nuclear binding, the dissociation constant was 0.1 nanomole per liter, and maximum binding was 104 femtomoles per milligram of protein. In competition studies, 25-hydroxyvitamin D3 was 300 times weaker than 1α,25-dihydroxyvitamin D3. The existence of high-affinity sites supports a physiologic role for 1α,25-dihydroxyvitamin D3 in the pituitary.
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U2 - 10.1126/science.6250221
DO - 10.1126/science.6250221
M3 - Article
C2 - 6250221
AN - SCOPUS:0019303475
SN - 0036-8075
VL - 209
SP - 1247
EP - 1249
JO - Science
JF - Science
IS - 4462
ER -