[11] Expression of chemokine RANTES and production of monoclonal antibodies

Alan M. Krensky*, Peter J. Nelson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

RANTES was first identified as a cDNA in a search for genes expressed late (3-5 days) after T-cell activation. Definition of RANTES function depended on the generation of protein. This chapter describes the various techniques used to make recombinant RANTES protein, to test its activity, and to generate monoclonal antibodies to assess RANTES protein cell distribution.

Original languageEnglish (US)
Pages (from-to)162-174
Number of pages13
JournalMethods in enzymology
Volume287
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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