1,25(OH)2D response to combined zinc and phosphorus depletion in rats

P. L. Kimmel*, D. W. Watkins, E. Slatopolsky, C. B. Langman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


To investigate the role of zinc depletion in the response of calcitriol to a single provocative stimulus, we studied vitamin D metabolites, parathyroid hormone (PTH), and mineral balance during phosphorus depletion in animals with different zinc nutritional status. Male Lewis rats (66) were pair fed normal phosphorus (NP), zinc-replete (+) or -deplete (-) diets for 2 wk. Thereafter, one half of each paired group underwent 1 wk of phosphorus depletion (LP). Zinc-deplete animals had lower plasma zinc, and phosphorus-deplete animals had lower plasma phosphorus concentrations than respective nutritionally replete controls. Plasma calcium, PTH, and 25-hydroxycholecalciferol concentrations were similar in all four groups at end of experiment. Mean plasma 1,25 (OH)2D concentration increased 80% from 35 ± 3 to 63 ± 9 pg/ml (P < 0.007) when values were compared before and after phosphorus restriction zinc-replete animals (LP + group) The 7.9% increase from 35 ± 4 to 38 ± 4 pg/ml in 1,25(OH)2D concentration in animals with combined zinc and phosphorus depletion (LP-) was not significant. External calcium balance, however, was maintained in the combined zinc and phosphorus-deplete group. We conclude that zinc depletion limits the increase in plasma 1,25(OH)2D concentration associated with phosphorus depletion. The mechanism is unknown but may involve an effect of zinc on renal 25(OH)D 1-α-hydroxylase synthetic activity.

Original languageEnglish (US)
Pages (from-to)E319-E326
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number3 22-3
StatePublished - 1990


  • Calcitriol
  • Mineral metabolism
  • Parathyroid hormone
  • Vitamin D

ASJC Scopus subject areas

  • General Medicine


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