TY - JOUR
T1 - 17-Allyamino-17-demethoxygeldanamycin and 17-NN-dimethyl ethylene diamine-geldanamycin have cytotoxic activity against multiple gynecologic cancer cell types
AU - Gossett, Dana R.
AU - Bradley, Megan S.
AU - Jin, Xiaohong
AU - Lin, Jiayuh
N1 - Funding Information:
Dr. Gossett is a SGI/NICHD Fellow of the Reproductive Scientist Development Program, funded through NIH grant #2K12HD00849 and a Wyeth-Ayherst Pharmaceuticals grant. Dr. Lin is supported in part by the Samuel Waxman Cancer Research Foundation and NIH R21 CA103877-01. We are grateful to Dr. Masato Nishida for providing the Ishikawa endometrial cancer cell line and Dr. Vicki Baker for the A2780 ovarian cancer cell line.
PY - 2005/2
Y1 - 2005/2
N2 - HSP90 is a cellular chaperone that is overexpressed in many cancers. HSP90 assists in proper folding of a variety of clients, many of which are oncoproteins. HSP90 has been shown to be elevated in endometrial, ovarian, and breast cancer. Furthermore, HSP90 is known to stabilize the oncoprotein Akt; disruptions of the Akt pathway are common in gynecologic malignancies. We sought to evaluate the effectiveness of HSP90 inhibitors in gynecologic cancer. We tested two HSP90 inhibitors, 17-AAG and 17-DMAG, against gynecologic cancer cell lines (four endometrial, one cervical, one ovarian, and one breast cancer line). We performed Western blots to determine effects of treatment on levels of HSP90 client proteins and PARP cleavage. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assays were used to assess cell viability, and flow cytometry to quantitate cell-cycle distribution and apoptosis. After treatment with 17-AAG or 17-DMAG, we detected no decrease in HSP90 levels. Levels of other oncoproteins did decrease with treatment: phosphorylated and total Akt, and Met. One cell line underwent G1 arrest, and five showed G2 arrest. All showed some level of apoptotic cell death, which was confirmed by detection of PARP cleavage. Sensitivity to the drugs varied among cell lines, ranging from 20% to 90% apoptosis after treatment. Our data suggest that 17-DMAG may be more potent than 17-AAG. HSP90 inhibitors are effective cytotoxic agents in gynecologic cancer cells. Further testing in in vivo model systems is warranted, with the goal of eventual translation to clinical trials in gynecologic oncology patients.
AB - HSP90 is a cellular chaperone that is overexpressed in many cancers. HSP90 assists in proper folding of a variety of clients, many of which are oncoproteins. HSP90 has been shown to be elevated in endometrial, ovarian, and breast cancer. Furthermore, HSP90 is known to stabilize the oncoprotein Akt; disruptions of the Akt pathway are common in gynecologic malignancies. We sought to evaluate the effectiveness of HSP90 inhibitors in gynecologic cancer. We tested two HSP90 inhibitors, 17-AAG and 17-DMAG, against gynecologic cancer cell lines (four endometrial, one cervical, one ovarian, and one breast cancer line). We performed Western blots to determine effects of treatment on levels of HSP90 client proteins and PARP cleavage. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assays were used to assess cell viability, and flow cytometry to quantitate cell-cycle distribution and apoptosis. After treatment with 17-AAG or 17-DMAG, we detected no decrease in HSP90 levels. Levels of other oncoproteins did decrease with treatment: phosphorylated and total Akt, and Met. One cell line underwent G1 arrest, and five showed G2 arrest. All showed some level of apoptotic cell death, which was confirmed by detection of PARP cleavage. Sensitivity to the drugs varied among cell lines, ranging from 20% to 90% apoptosis after treatment. Our data suggest that 17-DMAG may be more potent than 17-AAG. HSP90 inhibitors are effective cytotoxic agents in gynecologic cancer cells. Further testing in in vivo model systems is warranted, with the goal of eventual translation to clinical trials in gynecologic oncology patients.
KW - 17-AAG
KW - 17-DMAG
KW - AKT
KW - Endometrial cancer
KW - HSP90
KW - Molecular therapeutics
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UR - http://www.scopus.com/inward/citedby.url?scp=12344327270&partnerID=8YFLogxK
U2 - 10.1016/j.ygyno.2004.10.009
DO - 10.1016/j.ygyno.2004.10.009
M3 - Article
C2 - 15661225
AN - SCOPUS:12344327270
SN - 0090-8258
VL - 96
SP - 381
EP - 388
JO - Gynecologic Oncology
JF - Gynecologic Oncology
IS - 2
ER -