Abstract
SDS gel electrophoresis of microtubule proteins obtained from bovine brain by polymerization cycles revealed a new protein of 18 kDa. This protein was copolymerized with tubulin and its stoichiometry to tubulin remained constant for at least 5 cycles of assembly. Moreover, this protein remained bound to microtubules stabilized with 10 μM taxol and pelleted through a 4 M glycerol cushion. The same 18 kDa protein was found in a purified preparation of the high molecular mass microtubule-associated protein 1 (MAP-1). The 18 kDa protein copurified with the MAP-1 heavy chains during column chromatography on phosphocellulose, DEAE-cellulose, hydroxyapatite and Bio-Gel A-15m. Incubation of the MAP-1 preparation with a mouse monoclonal antibody to the light chain 1 (LC-1) of MAP-1 and with a second precipitating antibody (a rabbit antibody to mouse IgG) immunoprecipitated from the solution all the known components of MAP-1 (heavy chains, LC-1, LC-2), as well as the 18 kDa protein. Immunoblotting showed, however, that this antibody does not interact directly with the 18 kDa protein. These results indicate that the 18 kDa protein forms a complex with all other components of MAP-1. This polypeptide, therefore, is a new light chain (LC-3) of M AP-1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-148 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 212 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 9 1987 |
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Keywords
- Immunoprecipitation
- Microtubule
- Microtubule-associated protein 1
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
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18 kDa microtubule-associated protein : identification as a new light chain (LC-3) of microtubule-associated protein 1 (MAP-1). / Kuznetsov, Sergei A.; Gelfand, Vladimir I.
In: FEBS Letters, Vol. 212, No. 1, 09.02.1987, p. 145-148.Research output: Contribution to journal › Article
TY - JOUR
T1 - 18 kDa microtubule-associated protein
T2 - identification as a new light chain (LC-3) of microtubule-associated protein 1 (MAP-1)
AU - Kuznetsov, Sergei A.
AU - Gelfand, Vladimir I.
PY - 1987/2/9
Y1 - 1987/2/9
N2 - SDS gel electrophoresis of microtubule proteins obtained from bovine brain by polymerization cycles revealed a new protein of 18 kDa. This protein was copolymerized with tubulin and its stoichiometry to tubulin remained constant for at least 5 cycles of assembly. Moreover, this protein remained bound to microtubules stabilized with 10 μM taxol and pelleted through a 4 M glycerol cushion. The same 18 kDa protein was found in a purified preparation of the high molecular mass microtubule-associated protein 1 (MAP-1). The 18 kDa protein copurified with the MAP-1 heavy chains during column chromatography on phosphocellulose, DEAE-cellulose, hydroxyapatite and Bio-Gel A-15m. Incubation of the MAP-1 preparation with a mouse monoclonal antibody to the light chain 1 (LC-1) of MAP-1 and with a second precipitating antibody (a rabbit antibody to mouse IgG) immunoprecipitated from the solution all the known components of MAP-1 (heavy chains, LC-1, LC-2), as well as the 18 kDa protein. Immunoblotting showed, however, that this antibody does not interact directly with the 18 kDa protein. These results indicate that the 18 kDa protein forms a complex with all other components of MAP-1. This polypeptide, therefore, is a new light chain (LC-3) of M AP-1.
AB - SDS gel electrophoresis of microtubule proteins obtained from bovine brain by polymerization cycles revealed a new protein of 18 kDa. This protein was copolymerized with tubulin and its stoichiometry to tubulin remained constant for at least 5 cycles of assembly. Moreover, this protein remained bound to microtubules stabilized with 10 μM taxol and pelleted through a 4 M glycerol cushion. The same 18 kDa protein was found in a purified preparation of the high molecular mass microtubule-associated protein 1 (MAP-1). The 18 kDa protein copurified with the MAP-1 heavy chains during column chromatography on phosphocellulose, DEAE-cellulose, hydroxyapatite and Bio-Gel A-15m. Incubation of the MAP-1 preparation with a mouse monoclonal antibody to the light chain 1 (LC-1) of MAP-1 and with a second precipitating antibody (a rabbit antibody to mouse IgG) immunoprecipitated from the solution all the known components of MAP-1 (heavy chains, LC-1, LC-2), as well as the 18 kDa protein. Immunoblotting showed, however, that this antibody does not interact directly with the 18 kDa protein. These results indicate that the 18 kDa protein forms a complex with all other components of MAP-1. This polypeptide, therefore, is a new light chain (LC-3) of M AP-1.
KW - Immunoprecipitation
KW - Microtubule
KW - Microtubule-associated protein 1
UR - http://www.scopus.com/inward/record.url?scp=0023156677&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023156677&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(87)81574-0
DO - 10.1016/0014-5793(87)81574-0
M3 - Article
C2 - 3803603
AN - SCOPUS:0023156677
VL - 212
SP - 145
EP - 148
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -