193 nm photodissociation of larger multiply-charged biomolecules

Ziqiang Guan, Neil L. Kelleher, Peter B. O'Connor, David J. Aaserud, Daniel P. Little, Fred W. McLafferty*

*Corresponding author for this work

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

In contrast to most ion dissociation methods, 193 nm ultraviolet photodissociation of electrosprayed melittin (2.8 kDa) and ubiquitin (8.6 kDa) molecular ions yields new c and z ions (backbone amine bond dissociation) that provide additional sequence information. Dissociation by collisions or infrared photons produce b and y ions; for cleavages between the same amino acids the c ion represents the addition of NH2 to the b ion, and z the loss of NH2 from the y ion, so that these ions can be differentiated by this ± 16.02 Da difference. However, 193 nm photodissociation of 12–29 kDa ions as yet does not give collectable product ions, and that of the very stable y182+ ion from ubiquitin only effects a side chain loss. 193 nm irradiation of negative ions of all-T 30-mer DNA appears to eject electrons; apparently this is the first observation of electron photodetachment from multiply-charged negative ions.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalInternational Journal of Mass Spectrometry and Ion Processes
Volume157-158
DOIs
StatePublished - Jan 1 1996

Fingerprint

Photodissociation
Biomolecules
photodissociation
Ions
ions
dissociation
negative ions
Ubiquitin
Negative ions
Melitten
photodetachment
Electrons
molecular ions
amino acids
cleavage
amines
Amines
electrons
Amino acids
deoxyribonucleic acid

Keywords

  • 193 nm
  • Electrospray
  • Fourier transform ion cyclotron resonance
  • Photodissociation

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Guan, Ziqiang ; Kelleher, Neil L. ; O'Connor, Peter B. ; Aaserud, David J. ; Little, Daniel P. ; McLafferty, Fred W. / 193 nm photodissociation of larger multiply-charged biomolecules. In: International Journal of Mass Spectrometry and Ion Processes. 1996 ; Vol. 157-158. pp. 357-364.
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193 nm photodissociation of larger multiply-charged biomolecules. / Guan, Ziqiang; Kelleher, Neil L.; O'Connor, Peter B.; Aaserud, David J.; Little, Daniel P.; McLafferty, Fred W.

In: International Journal of Mass Spectrometry and Ion Processes, Vol. 157-158, 01.01.1996, p. 357-364.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 193 nm photodissociation of larger multiply-charged biomolecules

AU - Guan, Ziqiang

AU - Kelleher, Neil L.

AU - O'Connor, Peter B.

AU - Aaserud, David J.

AU - Little, Daniel P.

AU - McLafferty, Fred W.

PY - 1996/1/1

Y1 - 1996/1/1

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AB - In contrast to most ion dissociation methods, 193 nm ultraviolet photodissociation of electrosprayed melittin (2.8 kDa) and ubiquitin (8.6 kDa) molecular ions yields new c and z ions (backbone amine bond dissociation) that provide additional sequence information. Dissociation by collisions or infrared photons produce b and y ions; for cleavages between the same amino acids the c ion represents the addition of NH2 to the b ion, and z the loss of NH2 from the y ion, so that these ions can be differentiated by this ± 16.02 Da difference. However, 193 nm photodissociation of 12–29 kDa ions as yet does not give collectable product ions, and that of the very stable y182+ ion from ubiquitin only effects a side chain loss. 193 nm irradiation of negative ions of all-T 30-mer DNA appears to eject electrons; apparently this is the first observation of electron photodetachment from multiply-charged negative ions.

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KW - Electrospray

KW - Fourier transform ion cyclotron resonance

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