Soluble Immune Response Suppressor (SIRS)

Thomas M. Aune; H. William Schnaper; Carl W. Pierce

doi:10.1016/S0076-6879(85)16031-3

Soluble Immune Response Suppressor (SIRS)

Thomas M. Aune, H. William Schnaper, Carl W. Pierce

Pediatrics

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

This chapter provides an overview of soluble immune response suppressor (SIRS). The lymphokine SIRS is a protein which exists as two species of 14,000 and 21,000 daltons and is produced by suppressor T lymphocytes. SIRS is activated or oxidized to SIRSox by macrophages in an H202-mediated reaction. SIRSox activity was originally referred to as macrophage-derived suppressor factor before the relationship between SIRS and SIRSox. The chapter further explains preparation of SIRSOX. Activated SIRS or SIRSox can be obtained from three sources: (1) reaction of SIRS with H202, (2) culturing SIRS with splenic adherent cells, or (3) culturing SIRS with the macrophage like cell line RAW264.The most important component governing production of SIRSox from the reaction of SIRS with H202 appears to be the presence of contaminating proteins in the SIRS preparation.

Original language	English (US)
Pages (from-to)	395-402
Number of pages	8
Journal	Methods in enzymology
Volume	116
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(85)16031-3
State	Published - Jan 1 1985

ASJC Scopus subject areas

Molecular Biology
Biochemistry

Access to Document

10.1016/S0076-6879(85)16031-3

Cite this

@article{4d1ea76e57f84ef59d4698bdec1e59a0,

title = "Soluble Immune Response Suppressor (SIRS)",

abstract = "This chapter provides an overview of soluble immune response suppressor (SIRS). The lymphokine SIRS is a protein which exists as two species of 14,000 and 21,000 daltons and is produced by suppressor T lymphocytes. SIRS is activated or oxidized to SIRSox by macrophages in an H202-mediated reaction. SIRSox activity was originally referred to as macrophage-derived suppressor factor before the relationship between SIRS and SIRSox. The chapter further explains preparation of SIRSOX. Activated SIRS or SIRSox can be obtained from three sources: (1) reaction of SIRS with H202, (2) culturing SIRS with splenic adherent cells, or (3) culturing SIRS with the macrophage like cell line RAW264.The most important component governing production of SIRSox from the reaction of SIRS with H202 appears to be the presence of contaminating proteins in the SIRS preparation.",

author = "Aune, {Thomas M.} and {William Schnaper}, H. and Pierce, {Carl W.}",

year = "1985",

month = jan,

day = "1",

doi = "10.1016/S0076-6879(85)16031-3",

language = "English (US)",

volume = "116",

pages = "395--402",

journal = "Methods in enzymology",

issn = "0076-6879",

publisher = "Academic Press Inc.",

number = "C",

}

TY - JOUR

T1 - Soluble Immune Response Suppressor (SIRS)

AU - Aune, Thomas M.

AU - William Schnaper, H.

AU - Pierce, Carl W.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - This chapter provides an overview of soluble immune response suppressor (SIRS). The lymphokine SIRS is a protein which exists as two species of 14,000 and 21,000 daltons and is produced by suppressor T lymphocytes. SIRS is activated or oxidized to SIRSox by macrophages in an H202-mediated reaction. SIRSox activity was originally referred to as macrophage-derived suppressor factor before the relationship between SIRS and SIRSox. The chapter further explains preparation of SIRSOX. Activated SIRS or SIRSox can be obtained from three sources: (1) reaction of SIRS with H202, (2) culturing SIRS with splenic adherent cells, or (3) culturing SIRS with the macrophage like cell line RAW264.The most important component governing production of SIRSox from the reaction of SIRS with H202 appears to be the presence of contaminating proteins in the SIRS preparation.

AB - This chapter provides an overview of soluble immune response suppressor (SIRS). The lymphokine SIRS is a protein which exists as two species of 14,000 and 21,000 daltons and is produced by suppressor T lymphocytes. SIRS is activated or oxidized to SIRSox by macrophages in an H202-mediated reaction. SIRSox activity was originally referred to as macrophage-derived suppressor factor before the relationship between SIRS and SIRSox. The chapter further explains preparation of SIRSOX. Activated SIRS or SIRSox can be obtained from three sources: (1) reaction of SIRS with H202, (2) culturing SIRS with splenic adherent cells, or (3) culturing SIRS with the macrophage like cell line RAW264.The most important component governing production of SIRSox from the reaction of SIRS with H202 appears to be the presence of contaminating proteins in the SIRS preparation.

UR - http://www.scopus.com/inward/record.url?scp=0022272550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022272550&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(85)16031-3

DO - 10.1016/S0076-6879(85)16031-3

M3 - Article

C2 - 3878921

AN - SCOPUS:0022272550

SN - 0076-6879

VL - 116

SP - 395

EP - 402

JO - Methods in enzymology

JF - Methods in enzymology

IS - C

ER -

Soluble Immune Response Suppressor (SIRS)

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this