Soluble Immune Response Suppressor (SIRS)

Thomas M. Aune, H. William Schnaper, Carl W. Pierce

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


This chapter provides an overview of soluble immune response suppressor (SIRS). The lymphokine SIRS is a protein which exists as two species of 14,000 and 21,000 daltons and is produced by suppressor T lymphocytes. SIRS is activated or oxidized to SIRSox by macrophages in an H202-mediated reaction. SIRSox activity was originally referred to as macrophage-derived suppressor factor before the relationship between SIRS and SIRSox. The chapter further explains preparation of SIRSOX. Activated SIRS or SIRSox can be obtained from three sources: (1) reaction of SIRS with H202, (2) culturing SIRS with splenic adherent cells, or (3) culturing SIRS with the macrophage like cell line RAW264.The most important component governing production of SIRSox from the reaction of SIRS with H202 appears to be the presence of contaminating proteins in the SIRS preparation.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalMethods in enzymology
Issue numberC
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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