2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source

S. Chen*, Tatjana Paunesku, Y. Yuan, J. Deng, Q. Jin, Y. P. Hong, D. J. Vine, B. Lai, C. Flachenecker, B. Hornberger, Keith Brister, Chris Jacobsen, Gayle E Woloschak, S. Vogt

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.

Original languageEnglish (US)
Title of host publicationXRM 2014
Subtitle of host publicationProceedings of the 12th International Conference on X-Ray Microscopy
EditorsMartin D. de Jonge, David J. Paterson, Christopher G. Ryan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413436
DOIs
StatePublished - Jan 28 2016
Event12th International Conference on X-Ray Microscopy, XRM 2014 - Melbourne, Australia
Duration: Oct 26 2014Oct 31 2014

Publication series

NameAIP Conference Proceedings
Volume1696
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other12th International Conference on X-Ray Microscopy, XRM 2014
CountryAustralia
CityMelbourne
Period10/26/1410/31/14

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ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Chen, S., Paunesku, T., Yuan, Y., Deng, J., Jin, Q., Hong, Y. P., Vine, D. J., Lai, B., Flachenecker, C., Hornberger, B., Brister, K., Jacobsen, C., Woloschak, G. E., & Vogt, S. (2016). 2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source. In M. D. de Jonge, D. J. Paterson, & C. G. Ryan (Eds.), XRM 2014: Proceedings of the 12th International Conference on X-Ray Microscopy [020028] (AIP Conference Proceedings; Vol. 1696). American Institute of Physics Inc.. https://doi.org/10.1063/1.4937522