2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source

S. Chen; T. Paunesku; Y. Yuan; J. Deng; Q. Jin; Y. P. Hong; D. J. Vine; B. Lai; C. Flachenecker; B. Hornberger; K. Brister; C. Jacobsen; G. E. Woloschak; S. Vogt

doi:10.1063/1.4937522

2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source

S. Chen^*, T. Paunesku, Y. Yuan, J. Deng, Q. Jin, Y. P. Hong, D. J. Vine, B. Lai, C. Flachenecker, B. Hornberger, K. Brister, C. Jacobsen, G. E. Woloschak, S. Vogt

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

7 Scopus citations

Abstract

Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.

Original language	English (US)
Title of host publication	XRM 2014
Subtitle of host publication	Proceedings of the 12th International Conference on X-Ray Microscopy
Editors	Martin D. de Jonge, David J. Paterson, Christopher G. Ryan
Publisher	American Institute of Physics Inc.
ISBN (Electronic)	9780735413436
DOIs	https://doi.org/10.1063/1.4937522
State	Published - Jan 28 2016
Event	12th International Conference on X-Ray Microscopy, XRM 2014 - Melbourne, Australia Duration: Oct 26 2014 → Oct 31 2014

Publication series

Name	AIP Conference Proceedings
Volume	1696
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Other

Other	12th International Conference on X-Ray Microscopy, XRM 2014
Country/Territory	Australia
City	Melbourne
Period	10/26/14 → 10/31/14

ASJC Scopus subject areas

General Physics and Astronomy

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10.1063/1.4937522

Cite this

Chen, S., Paunesku, T., Yuan, Y., Deng, J., Jin, Q., Hong, Y. P., Vine, D. J., Lai, B., Flachenecker, C., Hornberger, B., Brister, K., Jacobsen, C., Woloschak, G. E., & Vogt, S. (2016). 2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source. In M. D. de Jonge, D. J. Paterson, & C. G. Ryan (Eds.), XRM 2014: Proceedings of the 12th International Conference on X-Ray Microscopy Article 020028 (AIP Conference Proceedings; Vol. 1696). American Institute of Physics Inc.. https://doi.org/10.1063/1.4937522

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title = "2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source",

abstract = "Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.",

author = "S. Chen and T. Paunesku and Y. Yuan and J. Deng and Q. Jin and Hong, {Y. P.} and Vine, {D. J.} and B. Lai and C. Flachenecker and B. Hornberger and K. Brister and C. Jacobsen and Woloschak, {G. E.} and S. Vogt",

note = "Publisher Copyright: {\textcopyright} 2016 AIP Publishing LLC.; 12th International Conference on X-Ray Microscopy, XRM 2014 ; Conference date: 26-10-2014 Through 31-10-2014",

year = "2016",

month = jan,

day = "28",

doi = "10.1063/1.4937522",

language = "English (US)",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics Inc.",

editor = "{de Jonge}, {Martin D.} and Paterson, {David J.} and Ryan, {Christopher G.}",

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Chen, S, Paunesku, T, Yuan, Y, Deng, J, Jin, Q, Hong, YP, Vine, DJ, Lai, B, Flachenecker, C, Hornberger, B, Brister, K, Jacobsen, C , Woloschak, GE & Vogt, S 2016, 2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source. in MD de Jonge, DJ Paterson & CG Ryan (eds), XRM 2014: Proceedings of the 12th International Conference on X-Ray Microscopy., 020028, AIP Conference Proceedings, vol. 1696, American Institute of Physics Inc., 12th International Conference on X-Ray Microscopy, XRM 2014, Melbourne, Australia, 10/26/14. https://doi.org/10.1063/1.4937522

2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source. / Chen, S.; Paunesku, T.; Yuan, Y. et al.
XRM 2014: Proceedings of the 12th International Conference on X-Ray Microscopy. ed. / Martin D. de Jonge; David J. Paterson; Christopher G. Ryan. American Institute of Physics Inc., 2016. 020028 (AIP Conference Proceedings; Vol. 1696).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Chen, S.

AU - Paunesku, T.

AU - Yuan, Y.

AU - Deng, J.

AU - Jin, Q.

AU - Hong, Y. P.

AU - Vine, D. J.

AU - Lai, B.

AU - Flachenecker, C.

AU - Hornberger, B.

AU - Brister, K.

AU - Jacobsen, C.

AU - Woloschak, G. E.

AU - Vogt, S.

PY - 2016/1/28

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N2 - Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.

AB - Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.

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A2 - de Jonge, Martin D.

A2 - Paterson, David J.

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ER -

Chen S, Paunesku T, Yuan Y, Deng J, Jin Q, Hong YP et al. 2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source. In de Jonge MD, Paterson DJ, Ryan CG, editors, XRM 2014: Proceedings of the 12th International Conference on X-Ray Microscopy. American Institute of Physics Inc. 2016. 020028. (AIP Conference Proceedings). doi: 10.1063/1.4937522

2D/3D cryo X-ray fluorescence imaging at the bionanoprobe at the advanced photon source

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