3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy

Thomas J. Deerinck; Maryann E. Martone; Varda Lev-Ram; David P L Green; Roger Y. Tsien; David L. Spector; Sui Huang; Mark H. Ellisman

3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy

Thomas J. Deerinck^*, Maryann E. Martone, Varda Lev-Ram, David P L Green, Roger Y. Tsien, David L. Spector, Sui Huang, Mark H. Ellisman

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A technique which combines fluorescence photooxidation and intermediate-voltage electron microscopy, was used in samples with the halogenated fluorescein derivative eosin. Eosin-conjugated reagents were utilized for high resolution immunolocalization of proteins in the confocal microscope. Specimens were then examined under the electron microscope after photooxidation of diaminobenzidine.

Original language	English (US)
Title of host publication	Proceedings - Annual Meeting, Microscopy Society of America
Editors	G.W. Bailey, A.J. Garratt-Reed
Pages	164-165
Number of pages	2
State	Published - Dec 1 1994
Event	Proceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA Duration: Jul 31 1994 → Aug 5 1994

Other

Other	Proceedings of the 52nd Annual Meeting of the Microscopy Society of America
City	New Orleans, LA, USA
Period	7/31/94 → 8/5/94

ASJC Scopus subject areas

Engineering(all)

Cite this

Deerinck, T. J., Martone, M. E., Lev-Ram, V., Green, D. P. L., Tsien, R. Y., Spector, D. L., Huang, S., & Ellisman, M. H. (1994). 3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy. In G. W. Bailey, & A. J. Garratt-Reed (Eds.), Proceedings - Annual Meeting, Microscopy Society of America (pp. 164-165)

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title = "3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy",

abstract = "A technique which combines fluorescence photooxidation and intermediate-voltage electron microscopy, was used in samples with the halogenated fluorescein derivative eosin. Eosin-conjugated reagents were utilized for high resolution immunolocalization of proteins in the confocal microscope. Specimens were then examined under the electron microscope after photooxidation of diaminobenzidine.",

author = "Deerinck, {Thomas J.} and Martone, {Maryann E.} and Varda Lev-Ram and Green, {David P L} and Tsien, {Roger Y.} and Spector, {David L.} and Sui Huang and Ellisman, {Mark H.}",

year = "1994",

month = dec,

day = "1",

language = "English (US)",

pages = "164--165",

editor = "G.W. Bailey and A.J. Garratt-Reed",

booktitle = "Proceedings - Annual Meeting, Microscopy Society of America",

note = "Proceedings of the 52nd Annual Meeting of the Microscopy Society of America ; Conference date: 31-07-1994 Through 05-08-1994",

}

Deerinck, TJ, Martone, ME, Lev-Ram, V, Green, DPL, Tsien, RY, Spector, DL, Huang, S & Ellisman, MH 1994, 3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy. in GW Bailey & AJ Garratt-Reed (eds), Proceedings - Annual Meeting, Microscopy Society of America. pp. 164-165, Proceedings of the 52nd Annual Meeting of the Microscopy Society of America, New Orleans, LA, USA, 7/31/94.

3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy. / Deerinck, Thomas J.; Martone, Maryann E.; Lev-Ram, Varda et al.

Proceedings - Annual Meeting, Microscopy Society of America. ed. / G.W. Bailey; A.J. Garratt-Reed. 1994. p. 164-165.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - 3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy

AU - Deerinck, Thomas J.

AU - Martone, Maryann E.

AU - Lev-Ram, Varda

AU - Green, David P L

AU - Tsien, Roger Y.

AU - Spector, David L.

AU - Huang, Sui

AU - Ellisman, Mark H.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - A technique which combines fluorescence photooxidation and intermediate-voltage electron microscopy, was used in samples with the halogenated fluorescein derivative eosin. Eosin-conjugated reagents were utilized for high resolution immunolocalization of proteins in the confocal microscope. Specimens were then examined under the electron microscope after photooxidation of diaminobenzidine.

AB - A technique which combines fluorescence photooxidation and intermediate-voltage electron microscopy, was used in samples with the halogenated fluorescein derivative eosin. Eosin-conjugated reagents were utilized for high resolution immunolocalization of proteins in the confocal microscope. Specimens were then examined under the electron microscope after photooxidation of diaminobenzidine.

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UR - http://www.scopus.com/inward/citedby.url?scp=0028756704&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0028756704

SP - 164

EP - 165

BT - Proceedings - Annual Meeting, Microscopy Society of America

A2 - Bailey, G.W.

A2 - Garratt-Reed, A.J.

T2 - Proceedings of the 52nd Annual Meeting of the Microscopy Society of America

Y2 - 31 July 1994 through 5 August 1994

ER -

3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy

Abstract

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