3-dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage electron microscopy

Thomas J. Deerinck*, Maryann E. Martone, Varda Lev-Ram, David P L Green, Roger Y. Tsien, David L. Spector, Sui Huang, Mark H. Ellisman

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A technique which combines fluorescence photooxidation and intermediate-voltage electron microscopy, was used in samples with the halogenated fluorescein derivative eosin. Eosin-conjugated reagents were utilized for high resolution immunolocalization of proteins in the confocal microscope. Specimens were then examined under the electron microscope after photooxidation of diaminobenzidine.

Original languageEnglish (US)
Title of host publicationProceedings - Annual Meeting, Microscopy Society of America
EditorsG.W. Bailey, A.J. Garratt-Reed
Pages164-165
Number of pages2
StatePublished - Dec 1 1994
EventProceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA
Duration: Jul 31 1994Aug 5 1994

Other

OtherProceedings of the 52nd Annual Meeting of the Microscopy Society of America
CityNew Orleans, LA, USA
Period7/31/948/5/94

ASJC Scopus subject areas

  • Engineering(all)

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