3-Methoxy-4-hydroxyphenylethyleneglycol (MHPG) transport from the spinal cord during spinal subarachnoid perfusion

John A. Kessler; Joseph D. Fenstermacher; Clifford S. Patlak

doi:10.1016/0006-8993(76)90579-5

3-Methoxy-4-hydroxyphenylethyleneglycol (MHPG) transport from the spinal cord during spinal subarachnoid perfusion

John A. Kessler^*, Joseph D. Fenstermacher, Clifford S. Patlak

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Spinal subarachnoid perfusions of rhesus monkeys were performed to study the transport of 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) by the spinal cord. The perfusate contained [³H]MHPG and a reference extracellular space marker ([¹⁴C]sucrose or [¹⁴C]ethylenediaminetetraacetic acid-calcium complex). The mean (± S.D.) clearance of MHPG by the monkey spinal cord was 33 (± 4) μl/min. Analysis of serial sections of spinal tissue after each perfusion facilitated determination of several basic parameters of MHPG transport. MHPG has a spinal tissue distribution space of 70-80%. MHPG enters capillaries withing spinal tissus throughout the entire spinal cord. The mean capillary exchange half-time was 8.2 min ± 1.9 min. MHPG crosses cell membranes easily and diffuses through cells rapidly. The apparent tissue diffusion coefficient approximated the diffusion coefficient in water (8.5 × 10^-6 sq.cm/sec). The data suggests that CSF levels of MHPG are an uncertain indicator of norepinephrine metabolism and that lumbar CSF levels of MHPG reflect in large part spinal cord rather than brain metabolism.

Original language	English (US)
Pages (from-to)	131-141
Number of pages	11
Journal	Brain research
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(76)90579-5
State	Published - Jan 30 1976

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "3-Methoxy-4-hydroxyphenylethyleneglycol (MHPG) transport from the spinal cord during spinal subarachnoid perfusion",

abstract = "Spinal subarachnoid perfusions of rhesus monkeys were performed to study the transport of 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) by the spinal cord. The perfusate contained [3H]MHPG and a reference extracellular space marker ([14C]sucrose or [14C]ethylenediaminetetraacetic acid-calcium complex). The mean (± S.D.) clearance of MHPG by the monkey spinal cord was 33 (± 4) μl/min. Analysis of serial sections of spinal tissue after each perfusion facilitated determination of several basic parameters of MHPG transport. MHPG has a spinal tissue distribution space of 70-80%. MHPG enters capillaries withing spinal tissus throughout the entire spinal cord. The mean capillary exchange half-time was 8.2 min ± 1.9 min. MHPG crosses cell membranes easily and diffuses through cells rapidly. The apparent tissue diffusion coefficient approximated the diffusion coefficient in water (8.5 × 10-6 sq.cm/sec). The data suggests that CSF levels of MHPG are an uncertain indicator of norepinephrine metabolism and that lumbar CSF levels of MHPG reflect in large part spinal cord rather than brain metabolism.",

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N2 - Spinal subarachnoid perfusions of rhesus monkeys were performed to study the transport of 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) by the spinal cord. The perfusate contained [3H]MHPG and a reference extracellular space marker ([14C]sucrose or [14C]ethylenediaminetetraacetic acid-calcium complex). The mean (± S.D.) clearance of MHPG by the monkey spinal cord was 33 (± 4) μl/min. Analysis of serial sections of spinal tissue after each perfusion facilitated determination of several basic parameters of MHPG transport. MHPG has a spinal tissue distribution space of 70-80%. MHPG enters capillaries withing spinal tissus throughout the entire spinal cord. The mean capillary exchange half-time was 8.2 min ± 1.9 min. MHPG crosses cell membranes easily and diffuses through cells rapidly. The apparent tissue diffusion coefficient approximated the diffusion coefficient in water (8.5 × 10-6 sq.cm/sec). The data suggests that CSF levels of MHPG are an uncertain indicator of norepinephrine metabolism and that lumbar CSF levels of MHPG reflect in large part spinal cord rather than brain metabolism.

AB - Spinal subarachnoid perfusions of rhesus monkeys were performed to study the transport of 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) by the spinal cord. The perfusate contained [3H]MHPG and a reference extracellular space marker ([14C]sucrose or [14C]ethylenediaminetetraacetic acid-calcium complex). The mean (± S.D.) clearance of MHPG by the monkey spinal cord was 33 (± 4) μl/min. Analysis of serial sections of spinal tissue after each perfusion facilitated determination of several basic parameters of MHPG transport. MHPG has a spinal tissue distribution space of 70-80%. MHPG enters capillaries withing spinal tissus throughout the entire spinal cord. The mean capillary exchange half-time was 8.2 min ± 1.9 min. MHPG crosses cell membranes easily and diffuses through cells rapidly. The apparent tissue diffusion coefficient approximated the diffusion coefficient in water (8.5 × 10-6 sq.cm/sec). The data suggests that CSF levels of MHPG are an uncertain indicator of norepinephrine metabolism and that lumbar CSF levels of MHPG reflect in large part spinal cord rather than brain metabolism.

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3-Methoxy-4-hydroxyphenylethyleneglycol (MHPG) transport from the spinal cord during spinal subarachnoid perfusion

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