3D chromatin interactions organize Yan chromatin occupancy and repression at the even-skipped locus

Jemma L. Webber, Jie Zhang, Aaron Mitchell-Dick, Ilaria Rebay*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Long-range integration of transcriptional inputs is critical for gene expression, yet the mechanisms remain poorly understood. We investigated the molecular determinants that confer fidelity to expression of the heart identity gene even-skipped (eve). Targeted deletion of regions bound by the repressor Yan defined two novel enhancers that contribute repressive inputs to stabilize tissue-specific output from a third enhancer. Deletion of any individual enhancer reduced Yan occupancy at the other elements, impacting eve expression, cell fate specification, and cardiac function. These long-range interactions may be stabilized by three-dimensional chromatin contacts that we detected between the elements. Our work provides a new paradigm for chromatin-level integration of general repressive inputs with specific patterning information to achieve robust gene expression.

Original languageEnglish (US)
Pages (from-to)2293-2298
Number of pages6
JournalGenes and Development
Volume27
Issue number21
DOIs
StatePublished - Nov 1 2013

Funding

Keywords

  • Chromatin conformation
  • Drosophila
  • ETS transcription factor
  • Gene regulation
  • Heart development
  • Robustness

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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