3D tissue distribution patterns characterized by micro-CT

Amy J. Wagoner Johnson*, Abigail M. Wojtowicz, Jennifer G. Dellinger, Russ D. Jamison, Konstantin I. Ignatiev, Stuart R Stock

*Corresponding author for this work

Research output: Contribution to journalConference article

2 Citations (Scopus)

Abstract

Porous hydroxyapatite bone scaffolds containing Os-stained tissue were imaged for the first time using x-ray microcomputed tomography. Hydroxyapatite scaffolds are seeded with mouse D1ORL stem cells and cultured in vitro for 2 weeks. Samples are fixed using a multi-step OsO4 procedure in order to increase the x-ray attenuation contrast between the scaffold and the tissue. Using this fixation method, the tissue and the scaffold are distinguishable. Data are obtained in the form of an attenuation value for each volume element in the sample, and these data allow samples to be imaged in cross section in increments of 6μm without cutting the sample, or as three dimensional objects.

Original languageEnglish (US)
Article numberF5.18
Pages (from-to)65-67
Number of pages3
JournalMaterials Research Society Symposium Proceedings
VolumeEXS
Issue number1
StatePublished - Dec 1 2004
Event2003 MRS Fall Meeting - Boston, MA, United States
Duration: Dec 1 2003Dec 4 2003

Fingerprint

Tissue Scaffolds
distribution (property)
Durapatite
Scaffolds
Tissue
X rays
Stem cells
Hydroxyapatite
Tomography
Bone
attenuation
Forms (concrete)
stem cells
bones
mice
x rays
tomography
cross sections

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Wagoner Johnson, A. J., Wojtowicz, A. M., Dellinger, J. G., Jamison, R. D., Ignatiev, K. I., & Stock, S. R. (2004). 3D tissue distribution patterns characterized by micro-CT. Materials Research Society Symposium Proceedings, EXS(1), 65-67. [F5.18].
Wagoner Johnson, Amy J. ; Wojtowicz, Abigail M. ; Dellinger, Jennifer G. ; Jamison, Russ D. ; Ignatiev, Konstantin I. ; Stock, Stuart R. / 3D tissue distribution patterns characterized by micro-CT. In: Materials Research Society Symposium Proceedings. 2004 ; Vol. EXS, No. 1. pp. 65-67.
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Wagoner Johnson, AJ, Wojtowicz, AM, Dellinger, JG, Jamison, RD, Ignatiev, KI & Stock, SR 2004, '3D tissue distribution patterns characterized by micro-CT', Materials Research Society Symposium Proceedings, vol. EXS, no. 1, F5.18, pp. 65-67.

3D tissue distribution patterns characterized by micro-CT. / Wagoner Johnson, Amy J.; Wojtowicz, Abigail M.; Dellinger, Jennifer G.; Jamison, Russ D.; Ignatiev, Konstantin I.; Stock, Stuart R.

In: Materials Research Society Symposium Proceedings, Vol. EXS, No. 1, F5.18, 01.12.2004, p. 65-67.

Research output: Contribution to journalConference article

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T1 - 3D tissue distribution patterns characterized by micro-CT

AU - Wagoner Johnson, Amy J.

AU - Wojtowicz, Abigail M.

AU - Dellinger, Jennifer G.

AU - Jamison, Russ D.

AU - Ignatiev, Konstantin I.

AU - Stock, Stuart R

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N2 - Porous hydroxyapatite bone scaffolds containing Os-stained tissue were imaged for the first time using x-ray microcomputed tomography. Hydroxyapatite scaffolds are seeded with mouse D1ORL stem cells and cultured in vitro for 2 weeks. Samples are fixed using a multi-step OsO4 procedure in order to increase the x-ray attenuation contrast between the scaffold and the tissue. Using this fixation method, the tissue and the scaffold are distinguishable. Data are obtained in the form of an attenuation value for each volume element in the sample, and these data allow samples to be imaged in cross section in increments of 6μm without cutting the sample, or as three dimensional objects.

AB - Porous hydroxyapatite bone scaffolds containing Os-stained tissue were imaged for the first time using x-ray microcomputed tomography. Hydroxyapatite scaffolds are seeded with mouse D1ORL stem cells and cultured in vitro for 2 weeks. Samples are fixed using a multi-step OsO4 procedure in order to increase the x-ray attenuation contrast between the scaffold and the tissue. Using this fixation method, the tissue and the scaffold are distinguishable. Data are obtained in the form of an attenuation value for each volume element in the sample, and these data allow samples to be imaged in cross section in increments of 6μm without cutting the sample, or as three dimensional objects.

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Wagoner Johnson AJ, Wojtowicz AM, Dellinger JG, Jamison RD, Ignatiev KI, Stock SR. 3D tissue distribution patterns characterized by micro-CT. Materials Research Society Symposium Proceedings. 2004 Dec 1;EXS(1):65-67. F5.18.