5-Hydroxytryptamine-3 receptors modulate synaptic activity in the rat nucleus tractus solitarius in vitro

Steven R. Glaum; Penelope A. Brooks; K. Michael Spyer; Richard J. Miller

doi:10.1016/0006-8993(92)91162-8

5-Hydroxytryptamine-3 receptors modulate synaptic activity in the rat nucleus tractus solitarius in vitro

Steven R. Glaum^*, Penelope A. Brooks, K. Michael Spyer, Richard J. Miller

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Whole-cell patch clamp recordings were made from neurons in the rat nucleus tractus solitarius (NTS) in transverse brainstem slices. 5-Hydroxytryptamine (5-HT, 100 μM) and the selective 5-HT₃ receptor agonist 2-methyl-5-HT (2-CH₃-5-HT, 100 μM) depolarized 86% of NTS neurons at resting membrane potential (V_m). This response was resistant to tetrodotoxin (TTX) and Co²⁺ application. In addition, 2-CH₃-5-HT (500 nM-100 μM) increased the amplitude and frequency of both excitatory and inhibitory spontaneous synaptic potentials. This effect was also TTX-resistant, but was abolished by Co²⁺. The effects of 2-CH₃-5-HT on EPSPs and IPSPs evoked by electrical stimulation of the tractus solitarius (TS) were analyzed separately in the presence of bicuculline or 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), respectively. Concentrations of 2-CH₃-5-HT between 500 nM and 1 μM decreased the amplitude of evoked EPSPs and IPSPs with similar potency. The selective 5-HT₃ receptor antagonists ICS 205-930 (10 nM) and MDL 72222 (10 μM) reversibly blocked the effects of 2-CH₃-5-HT at all doses examined. It is concluded that 5-HT₃ receptors can mediate both pre- and postsynaptic responses in the NTS.

Original language	English (US)
Pages (from-to)	62-68
Number of pages	7
Journal	Brain research
Volume	589
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(92)91162-8
State	Published - Aug 28 1992

Keywords

Brain slice
Medulla oblongata
Nucleus tractus solitarii
Patch clamp
Serotonin

ASJC Scopus subject areas

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

Access to Document

10.1016/0006-8993(92)91162-8

Cite this

@article{60ac3422beb144f59bb5b70cfb8e85c7,

title = "5-Hydroxytryptamine-3 receptors modulate synaptic activity in the rat nucleus tractus solitarius in vitro",

abstract = "Whole-cell patch clamp recordings were made from neurons in the rat nucleus tractus solitarius (NTS) in transverse brainstem slices. 5-Hydroxytryptamine (5-HT, 100 μM) and the selective 5-HT3 receptor agonist 2-methyl-5-HT (2-CH3-5-HT, 100 μM) depolarized 86% of NTS neurons at resting membrane potential (Vm). This response was resistant to tetrodotoxin (TTX) and Co2+ application. In addition, 2-CH3-5-HT (500 nM-100 μM) increased the amplitude and frequency of both excitatory and inhibitory spontaneous synaptic potentials. This effect was also TTX-resistant, but was abolished by Co2+. The effects of 2-CH3-5-HT on EPSPs and IPSPs evoked by electrical stimulation of the tractus solitarius (TS) were analyzed separately in the presence of bicuculline or 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), respectively. Concentrations of 2-CH3-5-HT between 500 nM and 1 μM decreased the amplitude of evoked EPSPs and IPSPs with similar potency. The selective 5-HT3 receptor antagonists ICS 205-930 (10 nM) and MDL 72222 (10 μM) reversibly blocked the effects of 2-CH3-5-HT at all doses examined. It is concluded that 5-HT3 receptors can mediate both pre- and postsynaptic responses in the NTS.",

keywords = "Brain slice, Medulla oblongata, Nucleus tractus solitarii, Patch clamp, Serotonin",

author = "Glaum, {Steven R.} and Brooks, {Penelope A.} and {Michael Spyer}, K. and Miller, {Richard J.}",

year = "1992",

month = aug,

day = "28",

doi = "10.1016/0006-8993(92)91162-8",

language = "English (US)",

volume = "589",

pages = "62--68",

journal = "Brain research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - 5-Hydroxytryptamine-3 receptors modulate synaptic activity in the rat nucleus tractus solitarius in vitro

AU - Glaum, Steven R.

AU - Brooks, Penelope A.

AU - Michael Spyer, K.

AU - Miller, Richard J.

PY - 1992/8/28

Y1 - 1992/8/28

N2 - Whole-cell patch clamp recordings were made from neurons in the rat nucleus tractus solitarius (NTS) in transverse brainstem slices. 5-Hydroxytryptamine (5-HT, 100 μM) and the selective 5-HT3 receptor agonist 2-methyl-5-HT (2-CH3-5-HT, 100 μM) depolarized 86% of NTS neurons at resting membrane potential (Vm). This response was resistant to tetrodotoxin (TTX) and Co2+ application. In addition, 2-CH3-5-HT (500 nM-100 μM) increased the amplitude and frequency of both excitatory and inhibitory spontaneous synaptic potentials. This effect was also TTX-resistant, but was abolished by Co2+. The effects of 2-CH3-5-HT on EPSPs and IPSPs evoked by electrical stimulation of the tractus solitarius (TS) were analyzed separately in the presence of bicuculline or 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), respectively. Concentrations of 2-CH3-5-HT between 500 nM and 1 μM decreased the amplitude of evoked EPSPs and IPSPs with similar potency. The selective 5-HT3 receptor antagonists ICS 205-930 (10 nM) and MDL 72222 (10 μM) reversibly blocked the effects of 2-CH3-5-HT at all doses examined. It is concluded that 5-HT3 receptors can mediate both pre- and postsynaptic responses in the NTS.

AB - Whole-cell patch clamp recordings were made from neurons in the rat nucleus tractus solitarius (NTS) in transverse brainstem slices. 5-Hydroxytryptamine (5-HT, 100 μM) and the selective 5-HT3 receptor agonist 2-methyl-5-HT (2-CH3-5-HT, 100 μM) depolarized 86% of NTS neurons at resting membrane potential (Vm). This response was resistant to tetrodotoxin (TTX) and Co2+ application. In addition, 2-CH3-5-HT (500 nM-100 μM) increased the amplitude and frequency of both excitatory and inhibitory spontaneous synaptic potentials. This effect was also TTX-resistant, but was abolished by Co2+. The effects of 2-CH3-5-HT on EPSPs and IPSPs evoked by electrical stimulation of the tractus solitarius (TS) were analyzed separately in the presence of bicuculline or 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), respectively. Concentrations of 2-CH3-5-HT between 500 nM and 1 μM decreased the amplitude of evoked EPSPs and IPSPs with similar potency. The selective 5-HT3 receptor antagonists ICS 205-930 (10 nM) and MDL 72222 (10 μM) reversibly blocked the effects of 2-CH3-5-HT at all doses examined. It is concluded that 5-HT3 receptors can mediate both pre- and postsynaptic responses in the NTS.

KW - Brain slice

KW - Medulla oblongata

KW - Nucleus tractus solitarii

KW - Patch clamp

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=0026674765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026674765&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(92)91162-8

DO - 10.1016/0006-8993(92)91162-8

M3 - Article

C2 - 1422823

AN - SCOPUS:0026674765

SN - 0006-8993

VL - 589

SP - 62

EP - 68

JO - Brain research

JF - Brain research

IS - 1

ER -

5-Hydroxytryptamine-3 receptors modulate synaptic activity in the rat nucleus tractus solitarius in vitro

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this