A 1.5-megabase yeast artificial chromosome contig from human chromosome 10q11.2 connecting three genetic loci (RET, D10S94, and D10S102) closely linked to the MEN2A locus

Terry C. Lairmore, Shenshen Dou, James R. Howe, David Chi, Katrin Carlson, Rosalie Veile, Santosh K. Mishra, Samuel A. Wells, Helen Donis-Keller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

The genetic loci RET, D10S94, and D10S102 from human chromosome 10q11.2 are very closely linked to a locus responsible for the multiple endocrine neoplasia type 2 (MEN2A and MEN2B) and medullary thyroid carcinoma (MTC1) familial cancer syndromes. We have constructed a 1.5-megabase contig consisting of six genomic yeast artificial chromosome clones which include these loci and define their physical order. A critical crossover event has been identified within the map interval; this event places the MEN2A locus centromeric to D10S102 and defines the orientation of the physical map on the chromosome. The orientation of the contig and order of the markers are centromere-RET-D10S94-ZM0S702-telomere. In addition, a microsatellite repeat polymorphism with a heterozygosity of 71% at the RET locus and a restriction fragment length polymorphism with a heterozygosity of 42% detected by a A clone from the D10S94 locus have been developed for high-resolution genetic linkage mapping and predictive diagnostic testing. These data place three important markers on a contiguous physical map, narrow the MEN2 disease locus interval, and provide a framework for further candidate gene identification efforts. Placement of these genetic loci along a clone-based map and continued expansion of the contig will also facilitate efforts to determine the relationship of physical to genetic distance near the centromeres of human chromosomes.

Original languageEnglish (US)
Pages (from-to)492-496
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number2
DOIs
StatePublished - Jan 15 1993

Keywords

  • Genomic walking
  • Microsatellite polymorphisms
  • Multiple endocrine neoplasia type 2
  • Yeast artificial chromosome clones

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'A 1.5-megabase yeast artificial chromosome contig from human chromosome 10q11.2 connecting three genetic loci (RET, D10S94, and D10S102) closely linked to the MEN2A locus'. Together they form a unique fingerprint.

Cite this