A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat

Susan J. Marriott; Paul F. Lindholm; Kenneth M. Brown; Scott D. Gitlin; Janet F. Duvall; Michael F. Radonovich; John N. Brady

doi:10.1128/MCB.10.8.4192

A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX₁ with a responsive element in the viral long terminal repeat

Susan J. Marriott, Paul F. Lindholm, Kenneth M. Brown, Scott D. Gitlin, Janet F. Duvall, Michael F. Radonovich, John N. Brady^*

^*Corresponding author for this work

Pathology

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Abstract

The human T-cell leukemia/lymphoma virus type I (HTLV-I) trans activator, TAX₁, interacts indirectly with a TAX₁,-responsive element, TRE-2, located at positions -117 to -163 in the viral long terminal repeat. This report describes the characterization of a 36-kilodalton (kDa) protein identified in HeLa nuclear extract which mediates the interaction of TAX₁ with TRE-2. Purification of the protein was achieved by zinc chelate chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The renatured 36-kDa protein bound specifically to a TRE-2 oligonucleotide but not to nonfunctional base substitution mutant probes in a gel retardation assay. Renatured proteins of differing molecular weights were unable to form this complex. In addition, the 36-kDa protein specifically activated transcription from the HTLV-I promoter in vitro. Purified TAX₁ protein formed a complex with the TRE-2 oligonucleotide in the presence of the 36-kDa protein, suggesting that indirect interaction of TAX₁ with the viral long terminal repeat may be one of the mechanisms by which HTLV-I transcription is regulated.

Original language	English (US)
Pages (from-to)	4192-4201
Number of pages	10
Journal	Molecular and cellular biology
Volume	10
Issue number	8
DOIs	https://doi.org/10.1128/MCB.10.8.4192
State	Published - 1990

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Molecular Biology
Cell Biology

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Marriott, S. J., Lindholm, P. F., Brown, K. M., Gitlin, S. D., Duvall, J. F., Radonovich, M. F., & Brady, J. N. (1990). A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX₁ with a responsive element in the viral long terminal repeat. Molecular and cellular biology, 10(8), 4192-4201. https://doi.org/10.1128/MCB.10.8.4192

@article{605558a801154813a67dad84e8a94a00,

title = "A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX1 with a responsive element in the viral long terminal repeat",

abstract = "The human T-cell leukemia/lymphoma virus type I (HTLV-I) trans activator, TAX1, interacts indirectly with a TAX1,-responsive element, TRE-2, located at positions -117 to -163 in the viral long terminal repeat. This report describes the characterization of a 36-kilodalton (kDa) protein identified in HeLa nuclear extract which mediates the interaction of TAX1 with TRE-2. Purification of the protein was achieved by zinc chelate chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The renatured 36-kDa protein bound specifically to a TRE-2 oligonucleotide but not to nonfunctional base substitution mutant probes in a gel retardation assay. Renatured proteins of differing molecular weights were unable to form this complex. In addition, the 36-kDa protein specifically activated transcription from the HTLV-I promoter in vitro. Purified TAX1 protein formed a complex with the TRE-2 oligonucleotide in the presence of the 36-kDa protein, suggesting that indirect interaction of TAX1 with the viral long terminal repeat may be one of the mechanisms by which HTLV-I transcription is regulated.",

author = "Marriott, {Susan J.} and Lindholm, {Paul F.} and Brown, {Kenneth M.} and Gitlin, {Scott D.} and Duvall, {Janet F.} and Radonovich, {Michael F.} and Brady, {John N.}",

year = "1990",

doi = "10.1128/MCB.10.8.4192",

language = "English (US)",

volume = "10",

pages = "4192--4201",

journal = "Molecular and cellular biology",

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Marriott, SJ, Lindholm, PF, Brown, KM, Gitlin, SD, Duvall, JF, Radonovich, MF & Brady, JN 1990, 'A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX₁ with a responsive element in the viral long terminal repeat', Molecular and cellular biology, vol. 10, no. 8, pp. 4192-4201. https://doi.org/10.1128/MCB.10.8.4192

A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX₁ with a responsive element in the viral long terminal repeat. / Marriott, Susan J.; Lindholm, Paul F.; Brown, Kenneth M. et al.
In: Molecular and cellular biology, Vol. 10, No. 8, 1990, p. 4192-4201.

Research output: Contribution to journal › Article › peer-review

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AU - Marriott, Susan J.

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AU - Gitlin, Scott D.

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AU - Radonovich, Michael F.

AU - Brady, John N.

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N2 - The human T-cell leukemia/lymphoma virus type I (HTLV-I) trans activator, TAX1, interacts indirectly with a TAX1,-responsive element, TRE-2, located at positions -117 to -163 in the viral long terminal repeat. This report describes the characterization of a 36-kilodalton (kDa) protein identified in HeLa nuclear extract which mediates the interaction of TAX1 with TRE-2. Purification of the protein was achieved by zinc chelate chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The renatured 36-kDa protein bound specifically to a TRE-2 oligonucleotide but not to nonfunctional base substitution mutant probes in a gel retardation assay. Renatured proteins of differing molecular weights were unable to form this complex. In addition, the 36-kDa protein specifically activated transcription from the HTLV-I promoter in vitro. Purified TAX1 protein formed a complex with the TRE-2 oligonucleotide in the presence of the 36-kDa protein, suggesting that indirect interaction of TAX1 with the viral long terminal repeat may be one of the mechanisms by which HTLV-I transcription is regulated.

AB - The human T-cell leukemia/lymphoma virus type I (HTLV-I) trans activator, TAX1, interacts indirectly with a TAX1,-responsive element, TRE-2, located at positions -117 to -163 in the viral long terminal repeat. This report describes the characterization of a 36-kilodalton (kDa) protein identified in HeLa nuclear extract which mediates the interaction of TAX1 with TRE-2. Purification of the protein was achieved by zinc chelate chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The renatured 36-kDa protein bound specifically to a TRE-2 oligonucleotide but not to nonfunctional base substitution mutant probes in a gel retardation assay. Renatured proteins of differing molecular weights were unable to form this complex. In addition, the 36-kDa protein specifically activated transcription from the HTLV-I promoter in vitro. Purified TAX1 protein formed a complex with the TRE-2 oligonucleotide in the presence of the 36-kDa protein, suggesting that indirect interaction of TAX1 with the viral long terminal repeat may be one of the mechanisms by which HTLV-I transcription is regulated.

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A 36-kilodalton cellular transcription factor mediates an indirect interaction of human T-cell leukemia/lymphoma virus type I TAX₁ with a responsive element in the viral long terminal repeat

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