TY - JOUR
T1 - A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region
AU - Carpten, John D.
AU - Makalowska, Izabella
AU - Robbins, Christiane M.
AU - Scott, Nyasha
AU - Sood, Raman
AU - Connors, Tim D.
AU - Bonner, Tom I.
AU - Smith, Jeffrey R.
AU - Faruque, Mezbah U.
AU - Stephan, Dietrich A.
AU - Pinkett, Heather
AU - Morgenbesser, Sharon D.
AU - Su, Kui
AU - Graham, Chris
AU - Gregory, Simon G.
AU - Williams, Hawys
AU - McDonald, Louise
AU - Baxevanis, Andreas D.
AU - Klingler, Kathy W.
AU - Landes, Greg M.
AU - Trent, Jeffrey M.
N1 - Funding Information:
The authors thank G. Olayinka, H. Tinkle, J. Turner, S. McGee, M. Phipps, A. Duda, and L. Michalowsky for outstanding technical support. The authors recognize M. Warman and J. Marcelino for the CACP collaboration. This work was supported through the National Human Genome Research Institute Intramural Program.
PY - 2000/2/15
Y1 - 2000/2/15
N2 - Several hereditary disease loci have been genetically mapped to the chromosome 1q24-q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) contig of 1q25 by sequence and computational analysis, STS content mapping, and chromosome walking. One hundred thirty-six new STSs, including 10 novel simple sequence repeat polymorphisms that are being used for genetic refinement of multiple disease loci, have been generated from this contig and are shown to map to the 1q25 interval. The integrity of the 6-Mb BAC/PAC contig has been confirmed by restriction fingerprinting, and this contig is being used as a template for human chromosome 1 genome sequencing. A transcription mapping effort has resulted in the precise localization of 18 known genes and 31 ESTs by database searching, exon trapping, direct cDNA hybridization, and sample sequencing of BACs from the 1q25 contig. An additional 11 known genes and ESTs have been placed within the larger 1q24- q31 interval. These transcription units represent candidate genes for multiple hereditary diseases, including HPC1. (C) 2000 Academic Press.
AB - Several hereditary disease loci have been genetically mapped to the chromosome 1q24-q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) contig of 1q25 by sequence and computational analysis, STS content mapping, and chromosome walking. One hundred thirty-six new STSs, including 10 novel simple sequence repeat polymorphisms that are being used for genetic refinement of multiple disease loci, have been generated from this contig and are shown to map to the 1q25 interval. The integrity of the 6-Mb BAC/PAC contig has been confirmed by restriction fingerprinting, and this contig is being used as a template for human chromosome 1 genome sequencing. A transcription mapping effort has resulted in the precise localization of 18 known genes and 31 ESTs by database searching, exon trapping, direct cDNA hybridization, and sample sequencing of BACs from the 1q25 contig. An additional 11 known genes and ESTs have been placed within the larger 1q24- q31 interval. These transcription units represent candidate genes for multiple hereditary diseases, including HPC1. (C) 2000 Academic Press.
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U2 - 10.1006/geno.1999.6051
DO - 10.1006/geno.1999.6051
M3 - Article
C2 - 10708513
AN - SCOPUS:0034652490
SN - 0888-7543
VL - 64
SP - 1
EP - 14
JO - Genomics
JF - Genomics
IS - 1
ER -