A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region

John D. Carpten*, Izabella Makalowska, Christiane M. Robbins, Nyasha Scott, Raman Sood, Tim D. Connors, Tom I. Bonner, Jeffrey R. Smith, Mezbah U. Faruque, Dietrich A. Stephan, Heather Pinkett, Sharon D. Morgenbesser, Kui Su, Chris Graham, Simon G. Gregory, Hawys Williams, Louise McDonald, Andreas D. Baxevanis, Kathy W. Klingler, Greg M. LandesJeffrey M. Trent

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Several hereditary disease loci have been genetically mapped to the chromosome 1q24-q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) contig of 1q25 by sequence and computational analysis, STS content mapping, and chromosome walking. One hundred thirty-six new STSs, including 10 novel simple sequence repeat polymorphisms that are being used for genetic refinement of multiple disease loci, have been generated from this contig and are shown to map to the 1q25 interval. The integrity of the 6-Mb BAC/PAC contig has been confirmed by restriction fingerprinting, and this contig is being used as a template for human chromosome 1 genome sequencing. A transcription mapping effort has resulted in the precise localization of 18 known genes and 31 ESTs by database searching, exon trapping, direct cDNA hybridization, and sample sequencing of BACs from the 1q25 contig. An additional 11 known genes and ESTs have been placed within the larger 1q24- q31 interval. These transcription units represent candidate genes for multiple hereditary diseases, including HPC1. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalGenomics
Volume64
Issue number1
DOIs
StatePublished - Feb 15 2000

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region'. Together they form a unique fingerprint.

  • Cite this

    Carpten, J. D., Makalowska, I., Robbins, C. M., Scott, N., Sood, R., Connors, T. D., Bonner, T. I., Smith, J. R., Faruque, M. U., Stephan, D. A., Pinkett, H., Morgenbesser, S. D., Su, K., Graham, C., Gregory, S. G., Williams, H., McDonald, L., Baxevanis, A. D., Klingler, K. W., ... Trent, J. M. (2000). A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region. Genomics, 64(1), 1-14. https://doi.org/10.1006/geno.1999.6051