A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Edgar D. Goluch; Jwa Min Nam; Dimitra G. Georganopoulou; Thomas N. Chiesl; Kashan A. Shaikh; Kee S. Ryu; Annelise E. Barron; Chad A. Mirkin; Chang Liu

doi:10.1039/b606294f

A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Edgar D. Goluch, Jwa Min Nam, Dimitra G. Georganopoulou, Thomas N. Chiesl, Kashan A. Shaikh, Kee S. Ryu, Annelise E. Barron, Chad A. Mirkin, Chang Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

202 Scopus citations

Abstract

Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles - a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 μL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

Original language	English (US)
Pages (from-to)	1293-1299
Number of pages	7
Journal	Lab on a Chip
Volume	6
Issue number	10
DOIs	https://doi.org/10.1039/b606294f
State	Published - 2006

ASJC Scopus subject areas

Bioengineering
General Chemistry
Biochemistry
Biomedical Engineering

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title = "A bio-barcode assay for on-chip attomolar-sensitivity protein detection",

abstract = "Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles - a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 μL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.",

author = "Goluch, {Edgar D.} and Nam, {Jwa Min} and Georganopoulou, {Dimitra G.} and Chiesl, {Thomas N.} and Shaikh, {Kashan A.} and Ryu, {Kee S.} and Barron, {Annelise E.} and Mirkin, {Chad A.} and Chang Liu",

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AU - Goluch, Edgar D.

AU - Nam, Jwa Min

AU - Georganopoulou, Dimitra G.

AU - Chiesl, Thomas N.

AU - Shaikh, Kashan A.

AU - Ryu, Kee S.

AU - Barron, Annelise E.

AU - Mirkin, Chad A.

AU - Liu, Chang

PY - 2006

Y1 - 2006

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AB - Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles - a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 μL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

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A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Abstract

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