A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Edgar D. Goluch, Jwa Min Nam, Dimitra G. Georganopoulou, Thomas N. Chiesl, Kashan A. Shaikh, Kee S. Ryu, Annelise E. Barron, Chad A. Mirkin, Chang Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

186 Scopus citations


Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles - a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 μL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

Original languageEnglish (US)
Pages (from-to)1293-1299
Number of pages7
JournalLab on a Chip
Issue number10
StatePublished - 2006

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering


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