A bioactive self-assembled membrane to promote angiogenesis

Lesley W. Chow, Ronit Bitton, Matthew J. Webber, Daniel Carvajal, Kenneth R. Shull, Arun K. Sharma, Samuel I. Stupp*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

128 Scopus citations


We report here on a bioactive hierarchically structured membrane formed by self-assembly. The membrane is formed with hyaluronic acid and peptide amphiphiles with binding affinity for heparin, and its hierarchical structure contains both an amorphous zone and a layer of fibrils oriented perpendicular to the membrane plane. The design of bioactivity is based on the potential ability to bind and slowly release heparin-binding growth factors. Human mesenchymal stem cells (hMSCs) seeded on these membranes attached and remained viable. Basic fibroblast growth factor (FGF2) and vascular endothelial growth factor (VEGF) were incorporated within the membrane structure prior to self-assembly and released into media over a prolonged period of time (14 days). Using the chicken chorioallantoic membrane (CAM) assay, we also found that these membranes induced a significant and rapid enhancement of angiogenesis relative to controls.

Original languageEnglish (US)
Pages (from-to)1574-1582
Number of pages9
Issue number6
StatePublished - Feb 2011


  • Angiogenesis
  • Growth factors
  • Heparin
  • Hyaluronic acid
  • Membrane
  • Self-assembly

ASJC Scopus subject areas

  • Mechanics of Materials
  • Ceramics and Composites
  • Bioengineering
  • Biophysics
  • Biomaterials


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