A bioassay capable of measuring 1 picogram of 1, 25-dihydroxyvitamin D3

Paula H. Stern*, Alan J. Hamstra, Hector F. Deluca, Norman H. Bell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Fetal rat bones in organ culture constitute a sensitive system for assay of the vitamin D metabolite, 1α, 25-dihydroxyvitamin D3. Significant bone resorption is obtained with as little as 2 pg 1, 25-dihydroxyvitamin D3 after 48 h of culture and with 1 pg after 64 h of culture. In the current study, organ cultures of fetal rat bone are used as a bioassay for 1, 25-dihydroxyvitamin D in normal human plasma, which was prepared for assay by extraction with dichloromethane, chromatography on Sephadex LH-20, and purification on silicic acid by high pressure liquid chromatography. The concentration of 1α, 25-dihydroxyvitamin D3 in normal adult human plasma was 24.8 ± 2.0 pg/ml (n = 19) by this assay.

Original languageEnglish (US)
Pages (from-to)891-896
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Issue number6
StatePublished - Jun 1978

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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