A bioorganometallic approach for rapid electrochemical analysis of human immunodeficiency virus type-1 reverse transcriptase in serum

Mahmoud Labib, Patrick O. Shipman, Sanela Martić, Heinz Bernhard Kraatz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Determination of low-abundance human immunodeficiency virus (HIV) enzymes is essential for early detection of the viral infection. Designed for the rapid detection of the virus in human serum, a rapid and ultrasensitive electrochemical assay for HIV-1 reverse transcriptase (HIV-1 RT) is presented in this article. The assay format is based on the formation of a self-assembled monolayer (SAM) of a synthesized ferrocene (Fc)-labeled lipoic acid onto a gold electrode. X-ray photoelectron spectroscopy (XPS) and Time-of-Flight secondary ion mass spectrometry (ToF-SIMS) were employed to confirm the binding of the Fc-labeled lipoic acid to the gold via the Au-S bond. A short RT-specific peptide, VEAIIRILQQLLFIH, was covalently attached to the Fc-labeled lipoic acid. Square wave voltammetry (SWV) offered a two-dimensional measurement of RT based on the anodic shift and reduction of current density of the Fc redox signal upon binding of RT to its specific peptide. This allowed a linear quantification of the target RT in the range of 100-500 pg mL-1, equivalent to 85.5-427.4 fM, with a detection limit of 50 pg mL-1 (42.7 fM). The developed biosensor is inexpensive, easy to prepare and operate, and allows a highly selective detection of RT in 20 s.

Original languageEnglish (US)
Pages (from-to)5122-5128
Number of pages7
JournalElectrochimica Acta
Volume56
Issue number14
DOIs
StatePublished - May 30 2011
Externally publishedYes

Keywords

  • Bioorganometallics
  • Ferrocene
  • HIV-1 reverse transcriptase
  • Human serum
  • Square wave voltammetry

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

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