A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice

Monica M. Laronda, Alexandra L. Rutz, Shuo Xiao, Kelly A. Whelan, Francesca E. Duncan, Eric W. Roth, Teresa K. Woodruff, Ramille N. Shah

Research output: Contribution to journalArticle

  • 39 Citations

Abstract

Emerging additive manufacturing techniques enable investigation of the effects of pore geometry on cell behavior and function. Here, we 3D print microporous hydrogel scaffolds to test how varying pore geometry, accomplished by manipulating the advancing angle between printed layers, affects the survival of ovarian follicles. 30° and 60° scaffolds provide corners that surround follicles on multiple sides while 90° scaffolds have an open porosity that limits follicle-scaffold interaction. As the amount of scaffold interaction increases, follicle spreading is limited and survival increases. Follicle-seeded scaffolds become highly vascularized and ovarian function is fully restored when implanted in surgically sterilized mice. Moreover, pups are born through natural mating and thrive through maternal lactation. These findings present an in vivo functional ovarian implant designed with 3D printing, and indicate that scaffold pore architecture is a critical variable in additively manufactured scaffold design for functional tissue engineering.

LanguageEnglish (US)
Article number15261
JournalNature Communications
Volume8
DOIs
StatePublished - May 16 2017

Fingerprint

ovaries
Ovarian Follicle
Hydrogel
Porosity
Tissue Engineering
Lactation
Scaffolds
mice
Ovary
Mothers
porosity
tissue engineering
geometry
printing
3D printers
emerging
manufacturing
interactions
Geometry
Scaffolds (biology)

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

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abstract = "Emerging additive manufacturing techniques enable investigation of the effects of pore geometry on cell behavior and function. Here, we 3D print microporous hydrogel scaffolds to test how varying pore geometry, accomplished by manipulating the advancing angle between printed layers, affects the survival of ovarian follicles. 30° and 60° scaffolds provide corners that surround follicles on multiple sides while 90° scaffolds have an open porosity that limits follicle-scaffold interaction. As the amount of scaffold interaction increases, follicle spreading is limited and survival increases. Follicle-seeded scaffolds become highly vascularized and ovarian function is fully restored when implanted in surgically sterilized mice. Moreover, pups are born through natural mating and thrive through maternal lactation. These findings present an in vivo functional ovarian implant designed with 3D printing, and indicate that scaffold pore architecture is a critical variable in additively manufactured scaffold design for functional tissue engineering.",
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A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice. / Laronda, Monica M.; Rutz, Alexandra L.; Xiao, Shuo; Whelan, Kelly A.; Duncan, Francesca E.; Roth, Eric W.; Woodruff, Teresa K.; Shah, Ramille N.

In: Nature Communications, Vol. 8, 15261, 16.05.2017.

Research output: Contribution to journalArticle

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AU - Laronda,Monica M.

AU - Rutz,Alexandra L.

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AU - Duncan,Francesca E.

AU - Roth,Eric W.

AU - Woodruff,Teresa K.

AU - Shah,Ramille N.

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